Application of lentinan in suppression of Marek's disease virus infection

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2024-10-13 DOI:10.1016/j.psj.2024.104427

Qiming Gao , Kongda Zhu , Wanli Sun , Shun Li , Yixin Wang , Shuang Chang , Peng Zhao

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引用次数: 0

Abstract

Marek's disease virus (MDV) is an extremely widespread avian immunosuppressive virus. In recent years, many reports have shown that there are still cases of MDV infection and immunosuppression after immunization with the vaccine. Consequently, there is a need to develop alternative complementary approaches for strengthening the efficacy of MDV prevention and control measures. Lentinan (LNT) is a macromolecular compound with immune-enhancing activity extracted from shiitake mushrooms. To explore the value and effectiveness of administering LNT through drinking water in the prevention and control of MDV, this study first observed the effects of high and low doses of LNT on weight gain, organ development, viral replication, and antibody titer of an avian influenza virus subtype H9 (AIV-H9) inactivated vaccine in specific pathogen-free (SPF) chicks infected with the wild strain of MDV. The results showed that both high and low doses of LNT significantly alleviated the weight gain retardation and liver and spleen enlargement caused by MDV infection, and significantly inhibited the replication of MDV in SPF chicks (P < 0.05), compared with the MDV-positive control group, both high and low doses of LNT significantly increased the antibody titer of AIV-H9 after immunization with inactivated AIV-H9 vaccine (P < 0.0001). On this basis, we also observed the effects of a chicken Marek's disease meq gene deletion live vaccine (SC9-1 strain), administered alone or in combination with LNT, on MDV infections of varying virulence in Hy-Line Brown chicks. The results showed that combined administration of LNT and the SC9-1 vaccine resulted in a significant alleviation of weight gain retardation and liver and spleen enlargement due to MDV infection (P < 0.05), as well as a significant inhibition of MDV replication and release in Hy-Line Brown chicks compared to the vaccine alone (P < 0.0001). These findings suggest that LNT not only alleviates the adverse effects of MDV infection in chickens but also enhances the efficacy of MDV vaccination, offering a potential auxiliary measure for controlling MDV infection.

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在抑制马雷克氏病病毒感染方面的应用

马立克氏病病毒（MDV）是一种极为普遍的禽类免疫抑制病毒。近年来，许多报告显示，在接种疫苗后仍有感染 MDV 和免疫抑制的病例。因此，有必要开发其他补充方法，以加强 MDV 防控措施的效果。龙胆素（LNT）是从香菇中提取的一种具有免疫增强活性的大分子化合物。为了探索通过饮水添加 LNT 在 MDV 防控中的价值和效果，本研究首先观察了高剂量和低剂量 LNT 对感染 MDV 野毒株的特定无病原（SPF）雏鸡的增重、器官发育、病毒复制和禽流感病毒 H9 亚型（AIV-H9）灭活疫苗抗体滴度的影响。结果表明，与MDV阳性对照组相比，高、低剂量LNT均能显著缓解MDV感染引起的增重迟缓、肝脾肿大等症状，并能显著抑制MDV在SPF雏鸡体内的复制（P< 0.05）；与MDV阳性对照组相比，高、低剂量LNT均能显著提高AIV-H9灭活疫苗免疫后的抗体滴度（P< 0.0001）。在此基础上，我们还观察了鸡马立克氏病meq基因缺失活疫苗（SC9-1株）单独或与LNT联合使用对不同毒力的MDV感染的影响。结果表明，与单独使用疫苗相比，联合使用 LNT 和 SC9-1 疫苗可显著缓解 MDV 感染引起的增重迟缓和肝脾肿大（P < 0.05），并显著抑制 MDV 在 Hy-Line 褐羽雏鸡体内的复制和释放（P < 0.0001）。这些发现表明，LNT 不仅能减轻鸡感染 MDV 的不良影响，还能提高 MDV 疫苗接种的效果，为控制 MDV 感染提供了一种潜在的辅助措施。

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.