Pyramiding of multiple resistant genes of blast and bacterial blight diseases in the background of rice (Oryza sativa) mega variety BRRI dhan29

IF 5.4 Q1 PLANT SCIENCES

Current Plant Biology Pub Date : 2024-10-18 DOI:10.1016/j.cpb.2024.100400

Mohammad Abdul Latif , Sheikh Arafat Islam Nihad, Rakibul Hasan, Lutfur Rahman, Touhidur Rahman Anik, Amal Chandra Manidas, Mamunur Rashid, Mohammad Ashik Iqbal Khan, Rejwan Bhuiyan

{"title":"Pyramiding of multiple resistant genes of blast and bacterial blight diseases in the background of rice (Oryza sativa) mega variety BRRI dhan29","authors":"Mohammad Abdul Latif , Sheikh Arafat Islam Nihad, Rakibul Hasan, Lutfur Rahman, Touhidur Rahman Anik, Amal Chandra Manidas, Mamunur Rashid, Mohammad Ashik Iqbal Khan, Rejwan Bhuiyan","doi":"10.1016/j.cpb.2024.100400","DOIUrl":null,"url":null,"abstract":"<div><div>Blast and bacterial blight (BB) are the two major rice diseases in the world including Bangladesh. In this study, BB resistance genes (Xa21 and xa13) and blast resistance (Pi9 and Pb1) genes were pyramided into a mega variety, BRRI dhan29 through marker-assisted backcross breeding. IRBB58 was used as a BB-resistant donor and Pi9-US2, and Pb1-US2 were used as blast-resistant donors. Backcross was done between BRRI dhan29 and donor parents to develop BC3F1 population and then selfing was done to develop BC3F6 population. BC3F2 population was genotyped and phenotyped for segregation analysis and BC3F6 was evaluated for genotyping, phenotyping and morphological traits and yields. Chi-square analysis of BC3F2 data revealed that blast and BB resistance followed the single gene mendelian fashion (1:2:1 and 3:1). Two to four gene combinations were found in the advanced lines of the BC3F6 population. The yield of the advanced lines ranged from 6.42 (t ha−1) to 9.5 (t ha−1) and they showed resistant against blast and BB with mean disease scores ranging from 0.67 to 2.33 and 0.33–2.33, respectively. Finally, eight lines having four genes (xa13, Xa21, Pi9 and Pb1) were selected for multilocational (five locations) trials for yield performance and disease reaction. Mean yield data of eight advanced lines of all locations were varied from 6.48±0.15–8.38±0.11 t ha−1 and all the lines showed resistant reactions against blast (score 0.53–1) and BB (score 0.6–0.87) disease. The highest yield was found in BR (Path) 13800-BC3–224–12 (G28, 8.38±0.11 t ha−1) followed by BR (Path) 13800-BC3–134–252 (G26, 8.28±0.08 t ha−1) and BR (Path) 13800-BC3–136–115 (G12, 8.24±0.07 t ha−1). Pyramided advanced lines of this study could be released as BB and blast-resistant varieties or could be utilized as donor parents in resistant breeding.</div></div>","PeriodicalId":38090,"journal":{"name":"Current Plant Biology","volume":"40 ","pages":"Article 100400"},"PeriodicalIF":5.4000,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Plant Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214662824000823","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Blast and bacterial blight (BB) are the two major rice diseases in the world including Bangladesh. In this study, BB resistance genes (Xa21 and xa13) and blast resistance (Pi9 and Pb1) genes were pyramided into a mega variety, BRRI dhan29 through marker-assisted backcross breeding. IRBB58 was used as a BB-resistant donor and Pi9-US2, and Pb1-US2 were used as blast-resistant donors. Backcross was done between BRRI dhan29 and donor parents to develop BC₃F₁ population and then selfing was done to develop BC₃F₆ population. BC₃F₂ population was genotyped and phenotyped for segregation analysis and BC₃F₆ was evaluated for genotyping, phenotyping and morphological traits and yields. Chi-square analysis of BC₃F₂ data revealed that blast and BB resistance followed the single gene mendelian fashion (1:2:1 and 3:1). Two to four gene combinations were found in the advanced lines of the BC₃F₆ population. The yield of the advanced lines ranged from 6.42 (t ha⁻¹) to 9.5 (t ha⁻¹) and they showed resistant against blast and BB with mean disease scores ranging from 0.67 to 2.33 and 0.33–2.33, respectively. Finally, eight lines having four genes (xa13, Xa21, Pi9 and Pb1) were selected for multilocational (five locations) trials for yield performance and disease reaction. Mean yield data of eight advanced lines of all locations were varied from 6.48±0.15–8.38±0.11 t ha⁻¹ and all the lines showed resistant reactions against blast (score 0.53–1) and BB (score 0.6–0.87) disease. The highest yield was found in BR (Path) 13800-BC3–224–12 (G28, 8.38±0.11 t ha⁻¹) followed by BR (Path) 13800-BC3–134–252 (G26, 8.28±0.08 t ha⁻¹) and BR (Path) 13800-BC3–136–115 (G12, 8.24±0.07 t ha⁻¹). Pyramided advanced lines of this study could be released as BB and blast-resistant varieties or could be utilized as donor parents in resistant breeding.

查看原文本刊更多论文

水稻（Oryza sativa）超大型品种 BRRI dhan29 背景中抗稻瘟病和细菌性疫病的多抗性基因的金字塔结构

稻瘟病和细菌性枯萎病（BB）是包括孟加拉国在内的世界两大水稻病害。在这项研究中，通过标记辅助回交育种，将抗BB基因（Xa21和xa13）和抗稻瘟病基因（Pi9和Pb1）构建成一个巨型品种BRRI dhan29。IRBB58 用作抗 BB 供体，Pi9-US2 和 Pb1-US2 用作抗瘟供体。在 BRRI dhan29 和供体亲本之间进行回交，培育出 BC3F1 群体，然后进行自交，培育出 BC3F6 群体。对 BC3F2 群体进行了基因分型和表型分析，对 BC3F6 进行了基因分型、表型、形态性状和产量评估。对 BC3F2 数据的卡方分析表明，稻瘟病和 BB 抗性遵循单基因孟德尔模式（1:2:1 和 3:1）。在 BC3F6 群体的先进品系中发现了 2 至 4 个基因组合。高级品系的产量在 6.42（吨/公顷-1）到 9.5（吨/公顷-1）之间，对稻瘟病和 BB 表现出抗性，平均病害评分分别在 0.67 到 2.33 之间和 0.33 到 2.33 之间。最后，有四个基因（xa13、Xa21、Pi9 和 Pb1）的八个品系被选中进行多地（五个地点）产量和病害反应试验。所有地点的 8 个先进品系的平均产量数据在 6.48±0.15-8.38±0.11 吨/公顷-1 之间，所有品系都表现出对稻瘟病（0.53-1 分）和 BB 病（0.6-0.87 分）的抗性。产量最高的是 BR (Path) 13800-BC3-224-12（G28，8.38±0.11 吨/公顷-1），其次是 BR (Path) 13800-BC3-134-252（G26，8.28±0.08 吨/公顷-1）和 BR (Path) 13800-BC3-136-115（G12，8.24±0.07 吨/公顷-1）。本研究的金字塔型先进品系可作为抗 BB 和抗瘟疫品种发布，也可作为抗性育种的供体亲本。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Current Plant Biology Agricultural and Biological Sciences-Plant Science

CiteScore

10.90

自引率

1.90%

发文量

审稿时长

50 days

期刊介绍： Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.