Occurrence, Complete Genome Sequencing, and Development of Diagnostics for Black Pepper Virus F Infecting Black Pepper in India

IF 1.1 4区农林科学 Q3 PLANT SCIENCES

Journal of Phytopathology Pub Date : 2024-10-22 DOI:10.1111/jph.13418

P. Malavika, M. Greeshma, A. I. Bhat

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引用次数: 0

Abstract

The occurrence of black pepper virus F (BPVF) was identified for the first time from India, and its complete genome sequence was determined using overlapping fragments obtained through reverse transcription polymerase chain reaction (RT-PCR). The RNA 1 and RNA 2 of the Indian isolate of BPVF (BPVF-IND) contained 6376 and 3340 nucleotides potentially coding for proteins of 230.7 kDa and 114 kDa respectively. Comparison of the RNA 1 sequence of BPVF-IND with that of BPVF from Brazil (BPVF- BR-PA) and China (BPVF- ZYP-1) revealed an identity of 95% and 90%, respectively, while RNA 2 showed an identity of 96% and 90%. The phylogenetic analysis of the Pro-Pol region of RNA 1 and coat protein region of RNA 2 revealed close clustering of all three BPVF isolates well separated from other species of the genus, Fabavirus. Diagnostics assays based on the RT-PCR and RT-recombinase polymerase amplification (RT-RPA) were developed for the sensitive detection of the virus that will help in the identification and propagation of virus-free black pepper plants.

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印度黑胡椒感染黑胡椒病毒 F 的发生、全基因组测序和诊断方法的开发

通过反转录聚合酶链式反应（RT-PCR）获得的重叠片段确定了黑胡椒病毒 F（BPVF）的完整基因组序列。印度分离的 BPVF（BPVF-IND）的 RNA 1 和 RNA 2 分别含有 6376 和 3340 个核苷酸，可能分别编码 230.7 kDa 和 114 kDa 的蛋白质。将 BPVF-IND 的 RNA 1 序列与巴西的 BPVF（BPVF- BR-PA）和中国的 BPVF（BPVF- ZYP-1）的 RNA 1 序列进行比较，发现两者的同一性分别为 95% 和 90%，而 RNA 2 的同一性分别为 96% 和 90%。对 RNA 1 的 Pro-Pol 区域和 RNA 2 的衣壳蛋白区域进行的系统进化分析表明，所有三个 BPVF 分离物的聚类都很接近，与法巴病毒属的其他物种相距甚远。基于 RT-PCR 和 RT-recombinase 聚合酶扩增（RT-RPA）技术开发的诊断方法可灵敏地检测病毒，有助于鉴定和繁殖无病毒的黑胡椒植株。

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来源期刊

Journal of Phytopathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.