Impact of HHIP gene polymorphisms on phenotypes, serum IL-17 and IL-18 in COPD patients of the Chinese Han population.

IF 5.8 2区 医学 Q1 Medicine
Jiajun Zhang, Di Zhao, Lili Zhang, Xueyan Feng, Beibei Li, Hui Dong, Yanchao Qi, Zun Jia, Fuyun Liu, Shaohui Zhao, Jin Zhang
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引用次数: 0

Abstract

Background: Genetic factors, including the Hedgehog Interacting Protein (HHIP) gene, play a crucial role in Chronic Obstructive Pulmonary Disease (COPD) susceptibility. This study examines the association between HHIP gene polymorphisms and COPD susceptibility, phenotypes, and serum IL-17 and IL-18 levels in a Han Chinese population.

Methods: A case-control study was conducted with 300 COPD patients and 300 healthy controls in Chinese Han population. Participants underwent genotyping for HHIP gene polymorphisms, pulmonary function tests, and quantitative CT scans. DNA samples were sequenced using a custom chip targeting the HHIP gene. Serum IL-17 and IL-18 levels were measured by enzyme-linked immunosorbent assay. Associations between SNPs, COPD susceptibility, and phenotypes were analyzed using logistic and multiple linear regression models, adjusting for confounders.

Results: Our study identified the rs11100865 polymorphism in the HHIP gene as significantly associated with COPD susceptibility (OR 2.479, 95% CI 1.527-4.024, P = 2.39E-04) after screening 114 SNPs through rigorous quality control. Stratified analyses further indicated this association was particularly in individuals aged 60 or older. Serum levels of IL-17 and IL-18 were significantly elevated in COPD patients compared to controls, with rs11100865 showing a notable association with IL-18 levels (B = 49.654, SE = 19.627, P = 0.012). However, no significant associations were observed between rs11100865 and serum IL-17 levels, COPD-related imaging parameters, or clinical phenotypes.

Conclusion: This study identified a significant association between HHIP gene polymorphisms and COPD susceptibility in a Han Chinese population, with connections to inflammation, but found no significant associations between this SNP and COPD-related imaging or clinical phenotypes.

Trial registration: www.chictr.org.cn ID: ChiCTR2300071579 2023-05-18.

HHIP基因多态性对中国汉族慢性阻塞性肺病患者表型、血清IL-17和IL-18的影响
背景:包括刺猬互作蛋白(HHIP)基因在内的遗传因素在慢性阻塞性肺病(COPD)易感性中起着至关重要的作用。本研究探讨了汉族人群中 HHIP 基因多态性与 COPD 易感性、表型以及血清 IL-17 和 IL-18 水平之间的关系:方法:对中国汉族人群中的 300 名慢性阻塞性肺病患者和 300 名健康对照者进行了病例对照研究。参与者接受了 HHIP 基因多态性基因分型、肺功能测试和定量 CT 扫描。使用针对 HHIP 基因的定制芯片对 DNA 样本进行测序。血清IL-17和IL-18水平通过酶联免疫吸附试验测定。使用逻辑和多元线性回归模型分析了SNPs、慢性阻塞性肺病易感性和表型之间的关联,并对混杂因素进行了调整:结果:通过严格的质量控制筛选出 114 个 SNPs 后,我们的研究发现 HHIP 基因中的 rs11100865 多态性与 COPD 易感性显著相关(OR 2.479,95% CI 1.527-4.024,P = 2.39E-04)。分层分析进一步表明,这种关联在 60 岁或以上的人群中尤为明显。与对照组相比,慢性阻塞性肺病患者血清中的IL-17和IL-18水平明显升高,其中rs11100865与IL-18水平有显著关联(B = 49.654,SE = 19.627,P = 0.012)。然而,在rs11100865与血清IL-17水平、COPD相关影像学参数或临床表型之间没有观察到明显的关联:本研究发现,在汉族人群中,HHIP 基因多态性与慢性阻塞性肺病易感性之间存在显著关联,且与炎症有关,但未发现该 SNP 与慢性阻塞性肺病相关影像学或临床表型之间存在显著关联。试验注册:www.chictr.org.cn ID:ChiCTR2300071579 2023-05-18.
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来源期刊
Respiratory Research
Respiratory Research RESPIRATORY SYSTEM-
CiteScore
9.70
自引率
1.70%
发文量
314
审稿时长
4-8 weeks
期刊介绍: Respiratory Research publishes high-quality clinical and basic research, review and commentary articles on all aspects of respiratory medicine and related diseases. As the leading fully open access journal in the field, Respiratory Research provides an essential resource for pulmonologists, allergists, immunologists and other physicians, researchers, healthcare workers and medical students with worldwide dissemination of articles resulting in high visibility and generating international discussion. Topics of specific interest include asthma, chronic obstructive pulmonary disease, cystic fibrosis, genetics, infectious diseases, interstitial lung diseases, lung development, lung tumors, occupational and environmental factors, pulmonary circulation, pulmonary pharmacology and therapeutics, respiratory immunology, respiratory physiology, and sleep-related respiratory problems.
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