Production of bacteriocin-like inhibitory substance (BLIS) by Staphylococcus spp. isolates from dogs.

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Ilana Nascimento de Sousa Santos, Walter Lilenbaum, Daniela Sales Alviano Moreno, Maria do Carmo de Freire Bastos
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Abstract

In the present study, 39 canine isolates of Staphylococcus spp. were tested for antimicrobial substance (AMS) production. Seven AMS producers were identified, whose products exhibited a non-acidic character and a proteinaceous nature, therefore being considered bacteriocin-like inhibitory substances (BLIS). The producer strains of BLIS P1, P16 and I3 showed a broad spectrum of antimicrobial activity. Human, veterinary and plant pathogens, such as Listeria monocytogenes, Klebsiella pneumoniae, Staphylococcus spp. and Clavibacter michiganensis, were among the inhibited micro-organisms, suggesting the potential biotechnological application of these peptides. MALDI-TOF mass spectrometry and 16 S rDNA sequencing identified the producer strains of BLIS P1, P16 and I3 as Staphylococcus pseudintermedius P1, Staphylococcus schleiferi P16 and Staphylococcus pseudintermedius I3. The plasmid profile of these strains suggests that the BLIS production is linked to biosynthetic genes located on plasmids. PCR analyses revealed that BLIS P1, P16 and I3 are different from 11 staphylococcins already described in the literature and that their genomic DNAs do not carry the most prevalent staphylococcal enterotoxin genes. The highest levels of BLIS production were achieved after 18-24 h of growth of the producer strains in TSB medium. Moreover, BLIS P1 and I3 exhibited high resistance to temperature and pH variations, and BLIS P16 maintained 100% of its activity in almost all conditions tested. The characteristics associated with BLIS P1, P16 and I3 described in this work encourage further investigation of these substances, in addition to this study being the first report of BLIS production by a strain of S. schleiferi.

从狗身上分离出的葡萄球菌产生细菌素样抑制物质(BLIS)。
本研究对 39 株犬类葡萄球菌分离株进行了抗菌物质(AMS)生产测试。结果发现了 7 种 AMS 生产菌株,它们的产品具有非酸性和蛋白性,因此被认为是类细菌素抑制物质(BLIS)。BLIS的生产菌株P1、P16和I3显示出广谱的抗菌活性。被抑制的微生物包括人类、兽医和植物病原体,如李斯特菌、肺炎克雷伯菌、葡萄球菌属和密歇根棒状杆菌,这表明这些肽具有潜在的生物技术应用价值。通过 MALDI-TOF 质谱分析和 16 S rDNA 测序,确定 BLIS P1、P16 和 I3 的生产菌株分别为假中间葡萄球菌 P1、假中间葡萄球菌 Schleiferi P16 和假中间葡萄球菌 I3。这些菌株的质粒特征表明,BLIS 的产生与位于质粒上的生物合成基因有关。PCR 分析表明,BLIS P1、P16 和 I3 与文献中已描述的 11 种葡萄球菌毒素不同,它们的基因组 DNA 不携带最常见的葡萄球菌肠毒素基因。生产菌株在 TSB 培养基中生长 18-24 小时后,BLIS 的产量达到最高水平。此外,BLIS P1 和 I3 对温度和 pH 值的变化具有很强的抵抗力,BLIS P16 在几乎所有测试条件下都能保持 100% 的活性。本研究中描述的与 BLIS P1、P16 和 I3 相关的特性鼓励了对这些物质的进一步研究,此外,本研究也是关于裂头蝇菌株产生 BLIS 的首次报道。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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