Blood microbiota in HIV-infected and HIV-uninfected patients with suspected sepsis detected by metagenomic next-generation sequencing.

IF 3.4 3区医学 Q2 INFECTIOUS DISEASES

BMC Infectious Diseases Pub Date : 2024-10-28 DOI:10.1186/s12879-024-10009-8

Qianhui Chen, Zhong Chen, Yuting Tan, Songjie Wu, Shi Zou, Jie Liu, Shihui Song, Qian Du, Min Wang, Ke Liang

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引用次数: 0

Abstract

Background: Information on the comparison of blood microbiota between human immunodeficiency virus (HIV)-infected and HIV-uninfected patients with suspected sepsis by metagenomic next-generation sequencing (mNGS) is limited.

Methods: Retrospectively analysis was conducted in HIV-infected and HIV-uninfected patients with suspected sepsis at Changsha First Hospital (China) from March 2019 to August 2022. Patients who underwent blood mNGS testing were enrolled. The blood microbiota detected by mNGS were analyzed.

Results: A total of 233 patients with suspected sepsis who performed blood mNGS were recruited in this study, including 79 HIV-infected and 154 HIV-uninfected patients. Compared with HIV-uninfected patients, the proportions of mycobacterium (p = 0.001), fungus (p < 0.001) and viruses (p < 0.001) were significantly higher, while the proportion of bacteria (p = 0.001) was significantly lower in HIV-infected patients. The higher positive rates of non-tuberculous mycobacteriosis (NTM, p = 0.022), Pneumocystis jirovecii (P. jirovecii) (p = 0.014), Talaromyces marneffei (T. marneffei) (p < 0.001) and cytomegalovirus (CMV) (p < 0.001) were observed in HIV-infected patients, compared with HIV-uninfected patients. In addition, compared with HIV-uninfected patients, the constituent ratio of T. marneffei (p < 0.001) in the fungus spectrum were significantly higher, while the constituent ratios of Candida (p < 0.001) and Aspergillus (p = 0.001) were significantly lower in HIV-infected patients.

Conclusions: Significant differences in the blood microbiota profiles exist between HIV-infected and HIV-uninfected patients with suspected sepsis.

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通过元基因组新一代测序技术检测疑似败血症的 HIV 感染者和 HIV 未感染者的血液微生物群。

背景：通过元基因组下一代测序（mNGS）比较人类免疫缺陷病毒（HIV）感染者和未感染HIV的疑似败血症患者血液微生物区系的资料有限：方法：对2019年3月至2022年8月期间长沙市第一医院（中国）疑似败血症的HIV感染者和HIV未感染者进行回顾性分析。患者均接受了血液 mNGS 检测。对 mNGS 检测到的血液微生物群进行分析：本研究共招募了 233 例进行血液 mNGS 检测的疑似败血症患者，其中包括 79 例 HIV 感染者和 154 例 HIV 未感染者。与未感染 HIV 的患者相比，分枝杆菌（p = 0.001）、真菌（p 结论：HIV 感染者和未感染 HIV 的患者的血液微生物比例存在显著差异：疑似败血症的 HIV 感染者和 HIV 未感染者的血液微生物群谱存在显著差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Infectious Diseases 医学-传染病学

CiteScore

6.50

自引率

0.00%

发文量

860

审稿时长

3.3 months

期刊介绍： BMC Infectious Diseases is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of infectious and sexually transmitted diseases in humans, as well as related molecular genetics, pathophysiology, and epidemiology.