Multitarget Phytocomplex: Focus on Antibacterial Profiles of Grape Pomace and Sambucus ebulus L. Lyophilisates Against Extensively Drug-Resistant (XDR) Bacteria and In Vitro Antioxidative Power.

IF 4.3 2区医学 Q1 INFECTIOUS DISEASES

Antibiotics-Basel Pub Date : 2024-10-17 DOI:10.3390/antibiotics13100980

Vladimir S Kurćubić, Vesna Đurović, Slaviša B Stajić, Marko Dmitrić, Saša Živković, Luka V Kurćubić, Pavle Z Mašković, Jelena Mašković, Milan Mitić, Vladimir Živković, Vladimir Jakovljević

{"title":"Multitarget Phytocomplex: Focus on Antibacterial Profiles of Grape Pomace and Sambucus ebulus L. Lyophilisates Against Extensively Drug-Resistant (XDR) Bacteria and In Vitro Antioxidative Power.","authors":"Vladimir S Kurćubić, Vesna Đurović, Slaviša B Stajić, Marko Dmitrić, Saša Živković, Luka V Kurćubić, Pavle Z Mašković, Jelena Mašković, Milan Mitić, Vladimir Živković, Vladimir Jakovljević","doi":"10.3390/antibiotics13100980","DOIUrl":null,"url":null,"abstract":"Objectives: This study was conceived with the aim of translating the experience and knowledge of the research group into the design and creation of multi-active phytocomplex cocktails from lyophilised winery by-products (Grape Pomace-GP) and weeds (Sambucus ebulus L., Dwarf Elder-DE). Methods: Quantification of bioactive molecules was performed by high-performance liquid chromatography (HPLC) method. Results: In the extract obtained from lyophilised GP, the most dominant component that was quantified was petunidin-3-glucoside. Prominent compounds that were quantified in DE extract were cyanidin derivatives. The total number of microorganisms in lyophilisates is low, but some of them still survive lyophilisation. Antibacterial activity was determined by microdilution, the minimum inhibitory concentration (MIC) of the tested bacteria ranged from 0.78 mg/mL to 25.00 mg/mL. Antibacterial susceptibility testing (AST) revealed that Klebsiella spp. and Acinetobacter baumannii complex are extensively drug-resistant (XDR). Conclusions: The GP + DE cocktail showed very strong AB power against both tested XDR bacteria. The total phenolic content and antioxidative effect (determined spectrophotometrically) indicate their linear correlation.","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"13 10","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11505505/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antibiotics-Basel","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/antibiotics13100980","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives: This study was conceived with the aim of translating the experience and knowledge of the research group into the design and creation of multi-active phytocomplex cocktails from lyophilised winery by-products (Grape Pomace-GP) and weeds (Sambucus ebulus L., Dwarf Elder-DE). Methods: Quantification of bioactive molecules was performed by high-performance liquid chromatography (HPLC) method. Results: In the extract obtained from lyophilised GP, the most dominant component that was quantified was petunidin-3-glucoside. Prominent compounds that were quantified in DE extract were cyanidin derivatives. The total number of microorganisms in lyophilisates is low, but some of them still survive lyophilisation. Antibacterial activity was determined by microdilution, the minimum inhibitory concentration (MIC) of the tested bacteria ranged from 0.78 mg/mL to 25.00 mg/mL. Antibacterial susceptibility testing (AST) revealed that Klebsiella spp. and Acinetobacter baumannii complex are extensively drug-resistant (XDR). Conclusions: The GP + DE cocktail showed very strong AB power against both tested XDR bacteria. The total phenolic content and antioxidative effect (determined spectrophotometrically) indicate their linear correlation.

查看原文本刊更多论文

多目标植物复合物：重点研究葡萄渣和欧洲三叶草冻干物对广泛耐药（XDR）细菌的抗菌谱以及体外抗氧化能力。

研究目的本研究旨在将研究小组的经验和知识转化为从冻干酒厂副产品（葡萄果脯-GP）和杂草（Sambucus ebulus L.，矮接骨木-DE）中设计和创造多活性植物复合鸡尾酒。方法：采用高效液相色谱法（HPLC）对生物活性分子进行定量。结果在冻干 GP 提取物中，最主要的定量成分是矮牵牛苷-3-葡萄糖苷。在 DE 提取物中定量的主要化合物是青花素衍生物。冻干物中的微生物总数较少，但仍有部分微生物在冻干过程中存活下来。抗菌活性通过微量稀释法测定，受试细菌的最低抑菌浓度（MIC）在 0.78 mg/mL 至 25.00 mg/mL 之间。抗菌药物敏感性测试（AST）显示，克雷伯氏菌属和鲍曼不动杆菌复合菌具有广泛耐药性（XDR）。结论GP + DE 鸡尾酒对两种经测试的 XDR 细菌都有很强的 AB 能力。总酚含量和抗氧化效果（分光光度法测定）表明两者呈线性相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Antibiotics-Basel Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics

CiteScore

7.30

自引率

14.60%

发文量

1547

审稿时长

11 weeks

期刊介绍： Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.