Genotypic Characterisation and Antimicrobial Resistance of Extended-Spectrum β-Lactamase-Producing Escherichia coli in Humans, Animals, and the Environment from Lusaka, Zambia: Public Health Implications and One Health Surveillance.

IF 4.3 2区医学 Q1 INFECTIOUS DISEASES

Antibiotics-Basel Pub Date : 2024-10-10 DOI:10.3390/antibiotics13100951

Maisa Kasanga, Márió Gajdács, Walter Muleya, Odion O Ikhimiukor, Steward Mudenda, Maika Kasanga, Joseph Chizimu, Doreen Mainza Shempela, Benjamin Bisesa Solochi, Mark John Mwikisa, Kaunda Yamba, Cheryl P Andam, Raphael Chanda, Duncan Chanda, Geoffrey Kwenda

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引用次数: 0

Abstract

Background: Extended-spectrum β-lactamases (ESBL) in Escherichia coli are a serious concern due to their role in developing multidrug resistance (MDR) and difficult-to-treat infections.

Objective: This study aimed to identify ESBL-carrying E. coli strains from both clinical and environmental sources in Lusaka District, Zambia.

Methods: This cross-sectional study included 58 ESBL-producing E. coli strains from hospital inpatients, outpatients, and non-hospital environments. Antimicrobial susceptibility was assessed using the Kirby-Bauer disk diffusion method and the VITEK^® 2 Compact System, while genotypic analyses utilised the Illumina NextSeq 2000 sequencing platform.

Results: Among the strains isolated strains, phylogroup B2 was the most common, with resistant MLST sequence types including ST131, ST167, ST156, and ST69. ESBL genes such as bla_TEM-1B, bla_CTX-M,bla_OXA-1, bla_NDM-5, and bla_CMY were identified, with ST131 and ST410 being the most common. ST131 exhibited a high prevalence of bla_CTX-M-15 and resistance to fluoroquinolones. Clinical and environmental isolates carried bla_NDM-5 (3.4%), with clinical isolates showing a higher risk of carbapenemase resistance genes and the frequent occurrence of bla_CTX-M and bla_TEM variants, especially bla_CTX-M-15 in ST131.

Conclusions: This study underscores the public health risks of bla_CTX-M-15- and bla_NDM-5-carrying E. coli. The strengthening antimicrobial stewardship programmes and the continuous surveillance of AMR in clinical and environmental settings are recommended to mitigate the spread of resistant pathogens.

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赞比亚卢萨卡人、动物和环境中产扩展谱 β-乳酰胺酶大肠埃希氏菌的基因型特征和抗菌药耐药性：公共卫生影响和统一健康监测。

背景：大肠埃希菌中的广谱β-内酰胺酶（ESBL）因其在发展多药耐药性（MDR）和难以治疗的感染中的作用而备受关注：本研究旨在从赞比亚卢萨卡地区的临床和环境来源中鉴定携带 ESBL 的大肠埃希菌菌株：这项横断面研究包括来自医院住院病人、门诊病人和非医院环境的 58 株产 ESBL 大肠杆菌。采用柯比鲍尔磁盘扩散法和 VITEK® 2 Compact 系统评估抗菌药敏感性，并利用 Illumina NextSeq 2000 测序平台进行基因型分析：在分离出的菌株中，系统组 B2 最为常见，耐药 MLST 序列类型包括 ST131、ST167、ST156 和 ST69。发现了 blaTEM-1B、blaCTX-M、blaOXA-1、blaNDM-5 和 blaCMY 等 ESBL 基因，其中以 ST131 和 ST410 最为常见。ST131 中 blaCTX-M-15 的流行率很高，并且对氟喹诺酮类药物具有耐药性。临床和环境分离菌株携带 blaNDM-5（3.4%），临床分离菌株对碳青霉烯酶耐药基因的风险较高，并经常出现 blaCTX-M 和 blaTEM 变体，尤其是 ST131 中的 blaCTX-M-15：本研究强调了携带 blaCTX-M-15 和 blaNDM-5 的大肠杆菌的公共卫生风险。建议加强抗菌药物管理计划，并持续监测临床和环境中的 AMR，以减少耐药病原体的传播。

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来源期刊

Antibiotics-Basel Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics

CiteScore

7.30

自引率

14.60%

发文量

1547

审稿时长

11 weeks

期刊介绍： Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.