Antimicrobial and Cytotoxic Potential of Eucalyptus Essential Oil-Based Nanoemulsions for Mouthwashes Application.

IF 4.3 2区医学 Q1 INFECTIOUS DISEASES

Antibiotics-Basel Pub Date : 2024-10-08 DOI:10.3390/antibiotics13100942

Dione Glauco Batista, William Gustavo Sganzerla, Lysa Ribeiro da Silva, Yasmin Gabriele Schmitt Vieira, Aline R Almeida, Diogo Dominguini, Luciane Ceretta, Adriana Castro Pinheiro, Fabiano Cleber Bertoldi, Daniela Becker, Dachamir Hotza, Michael Ramos Nunes, Cleonice Gonçalves da Rosa, Anelise Viapiana Masiero

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引用次数: 0

Abstract

Objective: An eucalyptus essential oil-based nanoemulsion was produced and evaluated for its antimicrobial properties against Streptococcus mutans and its cytotoxicity in the surface mucous cells of rabbits. Methods: The essential oil-based nanoemulsion was synthesized with two species of eucalyptus-Eucalyptus citriodora and Eucalyptus globulus-followed by physicochemical characterization and the determination of antimicrobial activity and cell viability. Subsequently, the mouthwash formulations (fluoride and fluoride-free) were functionalized with the nanoemulsion, and their in vitro antimicrobial actions were evaluated against S. mutans. Results: The nanoemulsion presented an average particle size of around 100 nm, a polydispersity index close to 0.3, a zeta potential between -19 and -30 mV, a pH close to 7, a spherical shape, and a cell viability above 50%. The antimicrobial activity analysis showed that the nanoemulsion was effective in the control of S. mutans. The mouthwashes functionalized with the nanoemulsion also presented bacteriostatic and bactericidal properties. Conclusions: The bio-based material produced with eucalyptus essential oil presented adequate physicochemical characteristics, with the potential to be used as an innovative material in preventive dentistry, contributing to the maintenance of oral and systemic health.

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桉树精油纳米乳液在漱口水应用中的抗菌和细胞毒性潜力

目的：制备桉树精油纳米乳液，并评估其对变异链球菌的抗菌特性及其对兔子表面粘液细胞的细胞毒性。研究方法用两种桉树--柠檬桉和球叶桉--合成了基于精油的纳米乳液，然后进行了理化表征，并测定了抗菌活性和细胞活力。随后，用纳米乳液对漱口水配方（含氟和不含氟）进行了功能化，并评估了它们对突变杆状病毒的体外抗菌作用。结果显示纳米乳液的平均粒径约为 100 纳米，多分散指数接近 0.3，zeta 电位介于 -19 和 -30 mV 之间，pH 值接近 7，呈球形，细胞存活率超过 50%。抗菌活性分析表明，纳米乳液能有效地控制突变杆状病毒。纳米乳液功能化的漱口水还具有抑菌和杀菌特性。结论用桉树精油生产的生物基材料具有适当的理化特性，有潜力用作预防性牙科的创新材料，为维护口腔和全身健康做出贡献。

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来源期刊

Antibiotics-Basel Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics

CiteScore

7.30

自引率

14.60%

发文量

1547

审稿时长

11 weeks

期刊介绍： Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.