Effects of 4.9 GHz Radiofrequency Field Exposure on Brain Metabolomic and Proteomic Characterization in Mice.

IF 3.6 3区生物学 Q1 BIOLOGY

Biology-Basel Pub Date : 2024-10-10 DOI:10.3390/biology13100806

Xing Wang, Guiqiang Zhou, Jiajin Lin, Zhaowen Zhang, Tongzhou Qin, Ling Guo, Haonan Wang, Zhifei Huang, Guirong Ding

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Abstract

Electromagnetic exposure has become increasingly widespread, and its biological effects have received extensive attention. The purpose of this study was to explore changes in the metabolism profile of the brain and serum and to identify differentially expressed proteins in the brain after exposure to the 4.9 GHz radiofrequency (RF) field. C57BL/6 mice were randomly divided into a Sham group and an RF group, which were sham-exposed and continuously exposed to a 4.9 RF field for 35 d, 1 h/d, at an average power density (PD) of 50 W/m². After exposure, untargeted metabolomics and Tandem Mass Tags (TMT) quantitative proteomics were performed. We found 104 and 153 up- and down-regulated differentially expressed metabolites (DEMs) in the RF_Brain group and RF_Serum group, and the DEMs were significantly enriched in glycerophospholipid metabolism. Moreover, 10 up-regulated and 51 down-regulated differentially expressed proteins (DEPs) were discovered in the RF group. Functional correlation analysis showed that most DEMs and DEPs showed a significant correlation. These results suggested that 4.9 GHz exposure induced disturbance of metabolism in the brain and serum, and caused deregulation of proteins in the brain.

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4.9 GHz 射频场暴露对小鼠脑代谢组和蛋白质组特征的影响

电磁暴露越来越普遍，其生物效应也受到广泛关注。本研究的目的是探讨暴露于 4.9 GHz 射频（RF）场后大脑和血清代谢谱的变化，并识别大脑中不同表达的蛋白质。将 C57BL/6 小鼠随机分为假暴露组和射频组，假暴露组在平均功率密度 (PD) 为 50 W/m2 的 4.9 射频场中暴露 35 天，每天 1 小时。暴露后，进行了非靶向代谢组学研究和串联质量标签（TMT）定量蛋白质组学研究。我们在射频脑组和射频血清组分别发现了104个和153个上调和下调的差异表达代谢物（DEMs），这些DEMs明显富集于甘油磷脂代谢中。此外，在 RF 组还发现了 10 个上调和 51 个下调的差异表达蛋白（DEPs）。功能相关性分析表明，大多数 DEMs 和 DEPs 显示出显著的相关性。这些结果表明，暴露于 4.9 GHz 会导致大脑和血清中的新陈代谢紊乱，并引起大脑中蛋白质的失调。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biology-Basel Biological Science-Biological Science

CiteScore

5.70

自引率

4.80%

发文量

1618

审稿时长

11 weeks

期刊介绍： Biology (ISSN 2079-7737) is an international, peer-reviewed, quick-refereeing open access journal of Biological Science published by MDPI online. It publishes reviews, research papers and communications in all areas of biology and at the interface of related disciplines. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material.