A Low-Cost, Portable, Multi-Cancer Screening Device Based on a Ratio Fluorometry and Signal Correlation Technique.

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL
Abdulaziz S Alghamdi, Rabah W Aldhaheri
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Abstract

The autofluorescence of erythrocyte porphyrins has emerged as a potential method for multi-cancer early detection (MCED). With this method's dependence on research-grade spectrofluorometers, significant improvements in instrumentation are necessary to translate its potential into clinical practice, as with any promising medical technology. To fill this gap, in this paper, we present an automated ratio porphyrin analyzer for cancer screening (ARPA-CS), a low-cost, portable, and automated instrument for MCED via the ratio fluorometry of porphyrins. The ARPA-CS aims to facilitate cancer screening in an inexpensive, rapid, non-invasive, and reasonably accurate manner for use in primary clinics or at point of care. To accomplish this, the ARPA-CS uses an ultraviolet-excited optical apparatus for ratio fluorometry that features two photodetectors for detection at 590 and 630 nm. Additionally, it incorporates a synchronous detector for the precision measurement of signals based on the Walsh-ordered Walsh-Hadamard transform (WHT)w and circular shift. To estimate its single-photodetector capability, we established a linear calibration curve for the ARBA-CS exceeding four orders of magnitude with a linearity of up to 0.992 and a low detection limit of 0.296 µg/mL for riboflavin. The ARPA-CS also exhibited excellent repeatability (0.21%) and stability (0.60%). Moreover, the ratio fluorometry of three serially diluted standard solutions of riboflavin yielded a ratio of 0.4, which agrees with that expected based on the known emission spectra of riboflavin. Additionally, the ratio fluorometry of the porphyrin solution yielded a ratio of 49.82, which was ascribed to the predominant concentration of protoporphyrin IX in the brown eggshells, as confirmed in several studies. This study validates this instrument for the ratio fluorometry of porphyrins as a biomarker for MCED. Nevertheless, large and well-designed clinical trials are necessary to further elaborate more on this matter.

基于比率荧光测定法和信号相关技术的低成本、便携式、多种癌症筛查设备。
红细胞卟啉的自发荧光已成为一种潜在的多癌症早期检测(MCED)方法。由于这种方法依赖于研究级的分光荧光仪,因此要将其潜力转化为临床实践,就必须对仪器进行重大改进,就像任何有前途的医疗技术一样。为了填补这一空白,我们在本文中介绍了用于癌症筛查的自动卟啉比值分析仪(ARPA-CS),这是一种通过卟啉比值荧光测定法进行 MCED 的低成本、便携式和自动化仪器。ARPA-CS 旨在以廉价、快速、无创和合理准确的方式促进癌症筛查,供基层诊所或医疗点使用。为实现这一目标,ARPA-CS 使用紫外线激发光学仪器进行比率荧光测定,该仪器有两个光电探测器,分别在 590 和 630 纳米波长进行检测。此外,它还集成了一个同步探测器,用于根据沃尔什有序沃尔什-哈达玛德变换 (WHT)w 和圆周偏移精确测量信号。为了评估其单光电探测器的能力,我们为 ARBA-CS 建立了一条线性校准曲线,其线性度超过四个数量级,线性度高达 0.992,核黄素的检测限低至 0.296 µg/mL。ARPA-CS 还具有出色的重复性(0.21%)和稳定性(0.60%)。此外,对核黄素的三种连续稀释的标准溶液进行比率荧光测定,得出的比率为 0.4,这与根据已知的核黄素发射光谱预期的比率一致。此外,对卟啉溶液进行比率荧光测定得出的比率为 49.82,这是因为棕色蛋壳中主要含有原卟啉 IX,这一点已在多项研究中得到证实。这项研究验证了该仪器可将卟啉比率荧光测定法作为 MCED 的生物标志物。不过,还需要进行大规模和设计良好的临床试验,以进一步阐明这一问题。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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