Fluorescence Immunoassay of Prostate-Specific Antigen Using 3D Paddle Screw-Type Devices and Their Rotating System.

IF 4.9 3区 工程技术 Q1 CHEMISTRY, ANALYTICAL
Su Bin Han, Han Sol Kim, Young Ju Jo, Soo Suk Lee
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引用次数: 0

Abstract

In this paper, we present a sensitive and highly reproducible fluorescence immunosensor for detecting PSA in human serum. A unique feature of this study is that it uses creatively designed paddle screw-type devices and their custom-made rotating system for PSA immunoassay. The paddle screw devices were designed to maximize the surface-to-volume ratio over which the immunoassay reaction could occur to improve detection sensitivity. This paddle screw-based immunoassay offers an accessible and efficient method with a short analysis time of less than 30 min. Active rotation of the paddle screw plays a crucial role in fast and accurate analysis of PSA. Additionally, a paddle screw-based immunoassay and subsequent fluorescence detection using a custom prototype fluorescence detection system were compared to a typical well plate-based immunoassay system. Results of PSA detection in human serum showed that the detection sensitivity through the paddle screw-based analysis improved about five times compared to that with a well plate-based analysis.

利用三维螺旋桨式装置及其旋转系统对前列腺特异性抗原进行荧光免疫测定
本文介绍了一种灵敏度高、重复性好的荧光免疫传感器,用于检测人体血清中的 PSA。这项研究的独特之处在于,它使用了创新设计的螺旋桨式装置及其定制的旋转系统来进行 PSA 免疫测定。桨式螺杆装置的设计旨在最大限度地提高免疫测定反应的表面体积比,从而提高检测灵敏度。这种以螺旋桨为基础的免疫测定方法简便高效,分析时间短,不超过 30 分钟。螺旋桨的主动旋转在快速准确地分析 PSA 方面起着至关重要的作用。此外,还将基于桨螺杆的免疫测定以及随后使用定制原型荧光检测系统进行的荧光检测与典型的基于井板的免疫测定系统进行了比较。人体血清中 PSA 的检测结果表明,与基于孔板的分析方法相比,基于桨螺杆的分析方法的检测灵敏度提高了约五倍。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biosensors-Basel
Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry
CiteScore
6.60
自引率
14.80%
发文量
983
审稿时长
11 weeks
期刊介绍: Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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