Antimicrobial susceptibility of Clostridium botulinum group III field strains isolated in Europe from animal outbreaks

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology Pub Date : 2024-10-16 DOI:10.1016/j.vetmic.2024.110281

Benedetta Cordioli , Alessia Rizzardi , Angela Guolo , Tiziana Ferro , Cosetta Bacchin , Manuel Garbuio , Fabrizio Anniballi , Paola De Santis , Miriam Koene , Caroline Le Maréchal , Hanna Skarin , Christian Seyboldt , Luca Bano

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引用次数: 0

Abstract

Neurotoxins produced by Clostridium (C.) botulinum group III are responsible for the majority of botulism outbreaks occurring in animals and in this study we report the drug susceptibility of 71 field strains. The minimum inhibitory concentration (MIC) of 13 antimicrobials was established through the agar dilution method. The MIC₅₀ matched or differed for one or two dilutions from MIC₉₀ of the same antimicrobial, showing a unimodal distribution of the MIC values, irrespective of the geographical origin, the animal source and the toxinotype of the strain. Beta-lactams and rifampin showed the lowest MIC values, while gentamicin, polymyxin B and sulfamethoxazole showed the highest MICs. As for similar studies conducted in human botulism, the results could be helpful to avoid the administration of antimicrobials that could worsen the health condition of the affected animals and to develop selective media for the isolation of these fastidious anaerobes. Indeed, the isolation of the strain from affected animals and from environmental samples is important to perform epidemiological studies based on the genetic characterization and to produce tailor-made vaccines.

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欧洲从动物疫情中分离出的第三类肉毒梭状芽孢杆菌野外菌株的抗菌药敏感性。

肉毒梭状芽孢杆菌（C. botulinum）III群产生的神经毒素是导致动物肉毒中毒事件爆发的主要原因，在这项研究中，我们报告了71株野外菌株对药物的敏感性。通过琼脂稀释法确定了 13 种抗菌药物的最低抑菌浓度（MIC）。在一个或两个稀释度中，MIC50 与同一种抗菌素的 MIC90 相匹配或存在差异，显示出 MIC 值的单峰分布，与菌株的地理来源、动物来源和毒素类型无关。β-内酰胺类和利福平的 MIC 值最低，而庆大霉素、多粘菌素 B 和磺胺甲噁唑的 MIC 值最高。至于在人类肉毒中毒中进行的类似研究，其结果可能有助于避免使用可能会恶化患病动物健康状况的抗菌药，并有助于开发用于分离这些苛刻厌氧菌的选择性培养基。事实上，从患病动物和环境样本中分离菌株对于根据基因特征进行流行病学研究和生产定制疫苗非常重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.