Investigation of Antioxidant and Antimicrobial Properties of Sunda Porcupine's (Hystrix javanica, F.Cuvier, 1823) Quills Ethanolic Crude Extract.

IF 1.1 Q3 BIOLOGY
Tropical life sciences research Pub Date : 2024-10-01 Epub Date: 2024-10-07 DOI:10.21315/tlsr2024.35.3.1
Muhamad Arif Budiman, Pamungkas Rizki Ferdian, Tri Hadi Handayani, Rizki Rabeca Elfirta, Masrukhin, Herjuno Ari Nugroho, Ni Luh Putu Rischa Phadmachanty, Wartika Rosa Farida, Ardya Widyastuti, Dianita Dwi Sugiartanti
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引用次数: 0

Abstract

The Sunda porcupine (Hystrix javanica, F.Cuvier, 1823) is a rodent-mammal species native to Indonesia and is utilised in traditional medicine for the treatment of various ailments. Some ethnic communities in Indonesia have traditional beliefs regarding Sunda porcupine's quills, which are thought to relieve back pain and toothache. Despite this traditional knowledge, there is limited scientific research on the topic. The aim of this study was to identify active compound in an ethanolic crude extract of Sunda porcupine's quills, and to evaluate its antioxidant and antimicrobial properties. The antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH)-free radical scavenging assay while the antimicrobial activity was evaluated through microdilution resazurin assay. The total phenolic and flavonoid contents were also determined to support the antioxidant properties. The active compounds were identified using gas chromatography-mass spectrophotometer (GCMS) with the National Institute of Standards and Technology (NIST-11) library. The result showed that the extract possesses antioxidant properties (IC50 138.93 μg/mL) and antimicrobial properties against Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis), Pseudomonas aeruginosa (P. aeruginosa) and Candida albicans (C. albicans) (IC50 range 0.40 mg/mL-33.05 mg/mL). Total phenolic content (TPC) and total flavonoid content (TFC) were 27.29 ± 2.20 mgGAE/g and 27.09 ± 1.66 mgQE/g, respectively. A total of 24 active compounds from the crude extract were identified. As much as five compounds serve as antioxidant agents, including: butylated hydroxytoluene; eicosane; 1-iodo-hexadecane; methyl ester hexadecanoic acid; and L-(+)-ascorbic acid 2,6-dihexadecanoate. Furthermore, as much as 11 compounds serve as antimicrobial agents, including: tetradecane; pentadecane; 2-isopropyl-5-methyl-1-heptanol; hexadecane; butylated hydroxytoluene; eicosane; 1-iodo-hexadecane; methyl ester hexadecanoic acid; benzenepropanoic acid, 3,5-bis(1,1-dimethylethyl)-4-hydroxy-, methyl ester; L-(+)-ascorbic acid 2,6-dihexadecanoate; and octadecanoic acid. This study provides scientific validation for the use of the Sunda porcupine's quills in traditional medicine and highlights the potential for further research in animal bioprospecting.

巽他箭猪(Hystrix javanica, F.Cuvier, 1823)羽毛乙醇粗提物的抗氧化和抗菌特性研究。
巽他豪猪(Hystrix javanica,F.Cuvier,1823 年)是一种原产于印度尼西亚的啮齿类哺乳动物,在传统医学中被用来治疗各种疾病。印度尼西亚的一些民族社区对巽他豪猪的箭有传统信仰,认为它能缓解背痛和牙痛。尽管有这些传统知识,但有关这一主题的科学研究却十分有限。本研究旨在确定巽他豪猪刺乙醇粗提取物中的活性化合物,并评估其抗氧化和抗菌特性。抗氧化活性采用 1,1-二苯基-2-苦基肼(DPPH)自由基清除法进行评估,而抗菌活性则采用微量稀释法进行评估。此外,还测定了总酚和类黄酮的含量,以支持其抗氧化特性。使用气相色谱-质谱仪(GCMS)和美国国家标准与技术研究院(NIST-11)文库对活性化合物进行了鉴定。结果表明,该提取物具有抗氧化性(IC50 138.93 μg/mL),对大肠杆菌(E. coli)、金黄色葡萄球菌(S. aureus)、枯草杆菌(B. subtilis)、铜绿假单胞菌(P. aeruginosa)和白色念珠菌(C. albicans)具有抗菌性(IC50 范围为 0.40 mg/mL-33.05 mg/mL)。总酚含量(TPC)和总黄酮含量(TFC)分别为 27.29 ± 2.20 mgGAE/g 和 27.09 ± 1.66 mgQE/g。从粗提取物中共鉴定出 24 种活性化合物。多达 5 种化合物可作为抗氧化剂,包括:丁基羟基甲苯、二十烷、1-碘-十六烷、十六烷酸甲酯和 L-(+)-抗坏血酸 2,6-二十六烷酸酯。此外,多达 11 种化合物可用作抗菌剂,包括十四烷;十五烷;2-异丙基-5-甲基-1-庚醇;十六烷;丁基羟基甲苯;二十烷;1-碘-十六烷;十六烷酸甲酯;苯丙酸,3,5-双(1,1-二甲基乙基)-4-羟基,甲酯;L-(+)-抗坏血酸 2,6-二十六烷酸酯;以及十八酸。这项研究为在传统医学中使用巽他豪猪的箭 quill 提供了科学验证,并强调了在动物生物勘探方面开展进一步研究的潜力。
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来源期刊
CiteScore
2.60
自引率
0.00%
发文量
40
审稿时长
20 weeks
期刊介绍: Tropical Life Sciences Research (TLSR) formerly known as Journal of Bioscience seeks to publish relevant ideas and knowledge addressing vital life sciences issues in the tropical region. The Journal’s scope is interdisciplinary in nature and covers any aspects related to issues on life sciences especially from the field of biochemistry, microbiology, biotechnology and animal, plant, environmental, biomedical and pharmaceutical sciences. TLSR practices double blind peer review system to ensure and maintain the good quality of articles published in this journal. Two issues are published annually in printed and electronic form. TLSR also accepts review articles, experimental papers and short communications. The Chief Editor would like to invite researchers to use this journal as a mean to rapidly promote their research findings.
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