Field-Applicable Loop-Mediated Isothermal Amplification for the Detection of Seven Common Human Papillomavirus Subtypes.

IF 2.8 4区 医学 Q2 INFECTIOUS DISEASES
Hongyi Li, He Tan, Xiaona Lv, Zhiqiang Han, Yuxin Wang, Shijue Gao, Ruiqin Zhang, Xinxin Shen, Xuejun Ma, Yanqing Tie
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Abstract

Persistent HPV infection is a major risk factor for the subsequent development of cervical cancer. LAMP is simple and suitable for field detection in the resource-limited settings. In this study, hydroxy naphthol blue (HNB)-based visual LAMP and evagreen-based fluorescent LAMP coupled with a microfluidic chip (LAMP-chip) were established for the field detection of seven subtypes of HPV. The analytical sensitivity was 19-233 copies/reaction. The overall clinical sensitivity was 97.35% for visual LAMP and 98.23% for LAMP-chip. Both LAMP assays exhibited 100% specificity and were completed in less than 50 min. Additionally, both assays did not require complicated nucleic acid extraction and purification steps. A complete quality control monitoring system (including internal control, positive quality control and negative control) in the LAMP assays further ensured the credibility of the results. Our findings demonstrated that the proposed LAMP assays have the potential to be applied in the testing of common HPV DNA in field investigations (visual LAMP) or within communities and primary health centers (LAMP-chip).

用于检测七种常见人类乳头状瘤病毒亚型的现场适用环路介导等温扩增技术。
人类乳头瘤病毒(HPV)的持续感染是导致宫颈癌的主要风险因素。LAMP 方法简单,适合在资源有限的环境中进行现场检测。本研究建立了基于羟基萘酚蓝(HNB)的可视化 LAMP 和基于 evagreen 的荧光 LAMP,并将其与微流控芯片(LAMP-chip)相结合,用于现场检测七种亚型的 HPV。分析灵敏度为 19-233 拷贝/反应。视觉 LAMP 和 LAMP 芯片的总体临床灵敏度分别为 97.35% 和 98.23%。两种 LAMP 检测方法的特异性均为 100%,且在 50 分钟内完成。此外,这两种检测方法都不需要复杂的核酸提取和纯化步骤。LAMP 检测中完整的质量控制监测系统(包括内部控制、阳性质量控制和阴性控制)进一步确保了检测结果的可信度。我们的研究结果表明,建议的 LAMP 检测方法有潜力应用于现场调查(目视 LAMP)或社区和初级保健中心(LAMP-芯片)中常见 HPV DNA 的检测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Tropical Medicine and Infectious Disease
Tropical Medicine and Infectious Disease Medicine-Public Health, Environmental and Occupational Health
CiteScore
3.90
自引率
10.30%
发文量
353
审稿时长
11 weeks
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