Description of Pegethrix niliensis sp. nov., a Novel Cyanobacterium from the Nile River Basin, Egypt: A Polyphasic Analysis and Comparative Study of Related Genera in the Oculatellales Order.

IF 3.9 3区 医学 Q2 FOOD SCIENCE & TECHNOLOGY
Toxins Pub Date : 2024-10-21 DOI:10.3390/toxins16100451
Guilherme Scotta Hentschke, Zakaria Mohamed, Alexandre Campos, Vitor M Vasconcelos
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引用次数: 0

Abstract

In this paper, we examine the filamentous cyanobacterial strain NILCB16 and describe it as a new species within the genus Pegethrix. The original population was sampled from a mat growing in an irrigation canal in the Nile River, Egypt. Initially classified under Plectonema or Planktolyngbya, the strain is a potential producer of the toxins microcystin and β-N-Methylamino-L-Alanine (BMAA). Additionally, we reviewed the taxonomic relationships between the Oculatellales genera. To describe the new species, we conducted a polyphasic study, encompassing 16S rRNA gene phylogenetic analyses performed using both Maximum Likelihood and Bayesian methods, sequence identity (p-distance) analysis, 16S-23S ITS secondary structures, and morphological and habitat comparisons. The phylogenetic analysis revealed that strain NILCB16 clustered within the Pegethrix clade with strong phylogenetic support, but in a distinct position from other species in the genus. The strain shared a maximum 16S rRNA gene identity of 97.3% with P. qiandaoensis and 96.1% with the type species, P. bostrychoides. Morphologically, NILCB16 can be differentiated from other species in the genus by its lack of false branching. Our phylogenetic analyses also show that Pegethrix, Cartusia, Elainella, and Maricoleus are clustered with strong phylogenetic support. They exhibit high 16S rRNA gene identity and are morphologically indistinguishable, suggesting they could potentially be merged into a single genus in the future.

描述来自埃及尼罗河流域的新型蓝藻--Pegethrix niliensis sp:多相分析及 Oculatellales 目相关属的比较研究。
本文研究了丝状蓝藻菌株 NILCB16,并将其描述为 Pegethrix 属中的一个新物种。其原始种群取自埃及尼罗河灌溉渠中生长的垫层。该菌株最初被归类为 Plectonema 或 Planktolyngbya,可能会产生毒素微囊藻毒素和 β-N-甲基氨基-L-丙氨酸(BMAA)。此外,我们还回顾了 Oculatellales 属之间的分类关系。为了描述这个新物种,我们进行了一项多相研究,包括使用最大似然法和贝叶斯法进行的 16S rRNA 基因系统进化分析、序列同一性(p-距离)分析、16S-23S ITS 二级结构以及形态和生境比较。系统进化分析表明,菌株 NILCB16 聚类于 Pegethrix 支系,具有较强的系统进化支持,但与该属其他物种的位置不同。该菌株的 16S rRNA 基因与 P. qiandaoensis 的最大同一性为 97.3%,与模式种 P. bostrychoides 的同一性为 96.1%。从形态上看,NILCB16 与该属其他物种的区别在于它没有假分支。我们的系统发育分析还显示,Pegethrix、Cartusia、Elainella 和 Maricoleus 在系统发育上有很强的聚类支持。它们表现出很高的 16S rRNA 基因同一性,而且在形态上没有区别,这表明它们将来有可能合并为一个属。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Toxins
Toxins TOXICOLOGY-
CiteScore
7.50
自引率
16.70%
发文量
765
审稿时长
16.24 days
期刊介绍: Toxins (ISSN 2072-6651) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to toxins and toxinology. It publishes reviews, regular research papers and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced.
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