Segatella clades adopt distinct roles within a single individual's gut.

IF 7.8 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

npj Biofilms and Microbiomes Pub Date : 2024-10-27 DOI:10.1038/s41522-024-00590-w

Xieyue Xiao, Adarsh Singh, Andrea Giometto, Ilana L Brito

引用次数: 0

Abstract

Segatella is a prevalent genus within individuals' gut microbiomes worldwide, especially in non-Western populations. Although metagenomic assembly and genome isolation have shed light on its genetic diversity, the lack of available isolates from this genus has resulted in a limited understanding of how members' genetic diversity translates into phenotypic diversity. Within the confines of a single gut microbiome, we have isolated 63 strains from diverse lineages of Segatella. We performed comparative analyses that exposed differences in cellular morphologies, preferences in polysaccharide utilization, yield of short-chain fatty acids, and antibiotic resistance across isolates. We further show that exposure to Segatella isolates either evokes strong or muted transcriptional responses in human intestinal epithelial cells. Our study exposes large phenotypic differences within related Segatella isolates, extending this to host-microbe interactions.

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Segatella 支系在单个个体的肠道中扮演着不同的角色。

Segatella属是世界范围内个体肠道微生物组中的一个普遍属，尤其是在非西方人群中。尽管元基因组组装和基因组分离揭示了其遗传多样性，但由于缺乏该属的分离株，人们对其成员的遗传多样性如何转化为表型多样性的了解十分有限。在单个肠道微生物组的范围内，我们分离出了 63 株来自 Segatella 不同品系的菌株。我们进行的比较分析揭示了不同分离株在细胞形态、多糖利用偏好、短链脂肪酸产量和抗生素耐药性方面的差异。我们进一步发现，暴露于 Segatella 分离物会在人类肠道上皮细胞中引起强烈或微弱的转录反应。我们的研究揭示了相关分离株中的巨大表型差异，并将其扩展到宿主与微生物之间的相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

npj Biofilms and Microbiomes Immunology and Microbiology-Microbiology

CiteScore

12.10

自引率

3.30%

发文量

审稿时长

9 weeks

期刊介绍： npj Biofilms and Microbiomes is a comprehensive platform that promotes research on biofilms and microbiomes across various scientific disciplines. The journal facilitates cross-disciplinary discussions to enhance our understanding of the biology, ecology, and communal functions of biofilms, populations, and communities. It also focuses on applications in the medical, environmental, and engineering domains. The scope of the journal encompasses all aspects of the field, ranging from cell-cell communication and single cell interactions to the microbiomes of humans, animals, plants, and natural and built environments. The journal also welcomes research on the virome, phageome, mycome, and fungome. It publishes both applied science and theoretical work. As an open access and interdisciplinary journal, its primary goal is to publish significant scientific advancements in microbial biofilms and microbiomes. The journal enables discussions that span multiple disciplines and contributes to our understanding of the social behavior of microbial biofilm populations and communities, and their impact on life, human health, and the environment.