A Mitochondria-Targeting Water-Soluble Fluorescent Probe for Selective Detection of Glyoxal in Living Cells.

IF 2.6 4区化学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Fluorescence Pub Date : 2024-10-23 DOI:10.1007/s10895-024-03994-1

Zhiming Wang, Chang Liu, Yuanyuan Wu, Huirong Yao, Song He, Liancheng Zhao, Xianshun Zeng

引用次数: 0

Abstract

Glyoxal (GL) is a physiological reactive α-oxoaldehyde metabolite, produced by lipid peroxidation and autoxidation of glucose. In this work, a specific mitochondria-targeting fluorescent probe Z-GL for glyoxal has been developed by an introducing isopropyl group on the recognition site to tune the selectivity toward glyoxal. The probe showed high selectivity and sensitivity for glyoxal in an aqueous system. Importantly, the probe was able to visualize exogenous and endogenous glyoxal in living cells. Furthermore, the probe was mitochondria-targetable, and could be used for monitoring the level of intracellular glyoxal.

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用于选择性检测活细胞中乙二醛的线粒体靶向水溶性荧光探针。

乙二醛（GL）是一种生理活性α-氧代醛代谢物，由脂质过氧化和葡萄糖自氧化产生。在这项工作中，通过在识别位点上引入异丙基以调节对乙二醛的选择性，开发出了一种特异性线粒体靶向乙二醛荧光探针 Z-GL。该探针在水体系中对乙二醛具有高选择性和灵敏度。重要的是，该探针能够检测活细胞中的外源性和内源性乙二醛。此外，该探针还具有线粒体靶向性，可用于监测细胞内乙二醛的水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Fluorescence 化学-分析化学

CiteScore

4.60

自引率

7.40%

发文量

203

审稿时长

5.4 months

期刊介绍： Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.