Pilot study on the impact of HIFU treatment on miRNA profiles in vaginal secretions of uterine fibroids and adenomyosis patients.

IF 3 3区 医学 Q2 ONCOLOGY
International Journal of Hyperthermia Pub Date : 2024-01-01 Epub Date: 2024-10-27 DOI:10.1080/02656736.2024.2418426
Wei-Chun Chen, Ting-Chang Chang, Lynn Perera, Mei-Hsiu Cheng, Jun-Jie Hong, Chao-Min Cheng
{"title":"Pilot study on the impact of HIFU treatment on miRNA profiles in vaginal secretions of uterine fibroids and adenomyosis patients.","authors":"Wei-Chun Chen, Ting-Chang Chang, Lynn Perera, Mei-Hsiu Cheng, Jun-Jie Hong, Chao-Min Cheng","doi":"10.1080/02656736.2024.2418426","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>High intensity focused ultrasound (HIFU) ablation treatment for uterine fibroids and adenomyosis has been long developed. The aim of this study is to investigate miRNA profile changes in vaginal secretions after HIFU treatment and their clinical relevance.</p><p><strong>Methods: </strong>We prospectively collected vaginal secretions samples from 8 patients (1 with adenomyosis and 7 with fibroids) before and after HIFU treatment. RNA was isolated and miRNA profiles were analyzed using next-generation sequencing (NGS) sequencing.</p><p><strong>Results: </strong>Our study showed miRNA profile change in vaginal secretion samples after HIFU treatment for uterine fibroids/adenomyosis, with 33 miRNAs upregulated and 6 downregulated overall. In fibroid cases, 31 miRNAs were upregulated and 7 downregulated, while in adenomyosis case, 41 miRNAs were upregulated and 71 downregulated. Four miRNAs (hsa-miR-7977, hsa-miR-155-5p, hsa-miR-191-5p, hsa-miR-223-3p) showed significant differences after HIFU treatment in fibroid cases, except in case 5 with the lowest treatment sonications (425 sonications) and energy input (170000 J). hsa-miR-7977 consistently showed downregulation after HIFU treatment. hsa-miR-155-5p were downregulated in case 4 with lowest treatment efficiency (2439.64 J/cm<sup>3</sup>), while they were upregulated in other cases. hsa-miR-191-5p and hsa-miR-223-3p were downregulated in cases 4 and 7, with case 7 influenced by high sonication and energy due to multiple fibroids.</p><p><strong>Conclusions: </strong>HIFU treatment altered miRNA profiles in fibroids/adenomyosis patients. Notably, hsa-miR-7977, hsa-miR-155-5p, hsa-miR-191-5p, and hsa-miR-223-3p showed significant changes in fibroid cases, except in low-energy treatments. hsa-miR-7977 consistently decreased post-treatment, while hsa-miR-155-5p decreased in the least efficient cases. Further research is needed for validation.</p>","PeriodicalId":14137,"journal":{"name":"International Journal of Hyperthermia","volume":null,"pages":null},"PeriodicalIF":3.0000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Hyperthermia","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/02656736.2024.2418426","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/27 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"ONCOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Background: High intensity focused ultrasound (HIFU) ablation treatment for uterine fibroids and adenomyosis has been long developed. The aim of this study is to investigate miRNA profile changes in vaginal secretions after HIFU treatment and their clinical relevance.

Methods: We prospectively collected vaginal secretions samples from 8 patients (1 with adenomyosis and 7 with fibroids) before and after HIFU treatment. RNA was isolated and miRNA profiles were analyzed using next-generation sequencing (NGS) sequencing.

Results: Our study showed miRNA profile change in vaginal secretion samples after HIFU treatment for uterine fibroids/adenomyosis, with 33 miRNAs upregulated and 6 downregulated overall. In fibroid cases, 31 miRNAs were upregulated and 7 downregulated, while in adenomyosis case, 41 miRNAs were upregulated and 71 downregulated. Four miRNAs (hsa-miR-7977, hsa-miR-155-5p, hsa-miR-191-5p, hsa-miR-223-3p) showed significant differences after HIFU treatment in fibroid cases, except in case 5 with the lowest treatment sonications (425 sonications) and energy input (170000 J). hsa-miR-7977 consistently showed downregulation after HIFU treatment. hsa-miR-155-5p were downregulated in case 4 with lowest treatment efficiency (2439.64 J/cm3), while they were upregulated in other cases. hsa-miR-191-5p and hsa-miR-223-3p were downregulated in cases 4 and 7, with case 7 influenced by high sonication and energy due to multiple fibroids.

Conclusions: HIFU treatment altered miRNA profiles in fibroids/adenomyosis patients. Notably, hsa-miR-7977, hsa-miR-155-5p, hsa-miR-191-5p, and hsa-miR-223-3p showed significant changes in fibroid cases, except in low-energy treatments. hsa-miR-7977 consistently decreased post-treatment, while hsa-miR-155-5p decreased in the least efficient cases. Further research is needed for validation.

关于 HIFU 治疗对子宫肌瘤和子宫腺肌症患者阴道分泌物中 miRNA 图谱影响的试点研究。
背景:高强度聚焦超声(HIFU)消融治疗子宫肌瘤和子宫腺肌症已经发展了很长时间。本研究旨在探讨 HIFU 治疗后阴道分泌物中 miRNA 的变化及其临床意义:方法:我们前瞻性地收集了 8 名患者(1 名腺肌症患者和 7 名子宫肌瘤患者)在 HIFU 治疗前后的阴道分泌物样本。方法:我们前瞻性地收集了 8 名患者(1 名腺肌症患者和 7 名子宫肌瘤患者)在 HIFU 治疗前后的阴道分泌物样本,并分离出 RNA,使用新一代测序技术(NGS)分析 miRNA 图谱:结果:我们的研究显示,子宫肌瘤/子宫腺肌症患者在接受 HIFU 治疗后,阴道分泌物样本中的 miRNA 发生了变化,总体上有 33 个 miRNA 上调,6 个下调。在子宫肌瘤病例中,31个miRNA上调,7个下调;在子宫腺肌症病例中,41个miRNA上调,71个下调。在子宫肌瘤病例中,有四个 miRNA(hsa-miR-7977、hsa-miR-155-5p、hsa-miR-191-5p 和 hsa-miR-223-3p)在 HIFU 治疗后出现了显著差异,但治疗超声次数(425 次)和能量输入(170000 焦耳)最低的病例 5 除外。hsa-miR-155-5p在治疗效率最低(2439.64 J/cm3)的病例4中下调,而在其他病例中上调。hsa-miR-191-5p和hsa-miR-223-3p在病例4和7中下调,其中病例7因多发性子宫肌瘤而受到高超声和高能量的影响:结论:HIFU治疗改变了子宫肌瘤/子宫腺肌症患者的miRNA谱。值得注意的是,hsa-miR-7977、hsa-miR-155-5p、hsa-miR-191-5p和hsa-miR-223-3p在子宫肌瘤病例中显示出显著变化,低能量治疗除外。还需要进一步研究验证。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
5.90
自引率
12.90%
发文量
153
审稿时长
6-12 weeks
期刊介绍: The International Journal of Hyperthermia
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信