BATF is involved in the malignant phenotype and epithelial-mesenchymal transition of colon cancer cells via ERK/PD-L1 signaling.

IF 2.5 4区生物学 Q3 CELL BIOLOGY

Histology and histopathology Pub Date : 2024-09-27 DOI:10.14670/HH-18-823

Xiaoqiong Chen, Huaqian Dong, Liping Jin

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引用次数: 0

Abstract

Objective: Transcription factors have emerged as primary regulators in colon cancer. Basic Leucine Zipper Transcription Factor (BATF) was found to be differentially expressed in colon cancer. This study aimed to explore the impact of BATF on the malignant phenotype and epithelial-mesenchymal transition (EMT) process.

Methods: Based on The Cancer Genome Atlas (TCGA) data, the correlation between BATF and patients' overall prognosis was analyzed. BATF expression in epithelial and colon cancer cells was evaluated. By knocking down its levels in colon cancer cells, its effects on the malignant phenotype, apoptosis, EMT progression, and ERK/PD-L1 were evaluated. Cells were treated with ERK/PD-L1 agonists, and the BATF cell regulation was re-examined.

Results: BATF levels were negatively correlated with patients' overall survival. BATF is upregulated in colon cancer cell lines, and BATF knockdown in HCT116 cells suppressed the malignant cellular phenotypes (proliferation, migration, and invasion) and increased apoptosis. BATF knockdown inhibited EMT and ERK/PD-L1 signaling activation, whereas upon agonist treatment, BATF potency was disrupted.

Conclusion: This study revealed that BATF is involved in the malignant phenotype and EMT of colon cancer cells, and this process may be mediated by ERK/PD-L1 signaling.

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BATF 通过 ERK/PD-L1 信号转导参与结肠癌细胞的恶性表型和上皮-间质转化。

目的：转录因子已成为结肠癌的主要调节因子。研究发现，碱性亮氨酸拉链转录因子（BATF）在结肠癌中有不同程度的表达。本研究旨在探讨 BATF 对恶性表型和上皮-间质转化（EMT）过程的影响：方法：基于癌症基因组图谱（TCGA）数据，分析BATF与患者总体预后的相关性。评估了 BATF 在上皮细胞和结肠癌细胞中的表达。通过降低 BATF 在结肠癌细胞中的水平，评估其对恶性表型、细胞凋亡、EMT 进展和 ERK/PD-L1 的影响。用ERK/PD-L1激动剂处理细胞，重新研究BATF对细胞的调控作用：结果：BATF水平与患者的总生存率呈负相关。BATF 在结肠癌细胞系中上调，敲除 HCT116 细胞中的 BATF 可抑制恶性细胞表型（增殖、迁移和侵袭）并增加细胞凋亡。BATF敲除抑制了EMT和ERK/PD-L1信号的激活，而在激动剂处理时，BATF的效力被破坏：本研究揭示了 BATF 参与了结肠癌细胞的恶性表型和 EMT，而这一过程可能是由 ERK/PD-L1 信号介导的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Histology and histopathology 生物-病理学

CiteScore

3.90

自引率

0.00%

发文量

232

审稿时长

2 months

期刊介绍： HISTOLOGY AND HISTOPATHOLOGY is a peer-reviewed international journal, the purpose of which is to publish original and review articles in all fields of the microscopical morphology, cell biology and tissue engineering; high quality is the overall consideration. Its format is the standard international size of 21 x 27.7 cm. One volume is published every year (more than 1,300 pages, approximately 90 original works and 40 reviews). Each volume consists of 12 numbers published monthly online. The printed version of the journal includes 4 books every year; each of them compiles 3 numbers previously published online.