The ubiquitin ligase VviPUB19 negatively regulates grape cold tolerance by affecting the stability of ICEs and CBFs

IF 8.7 1区农林科学 Q1 Agricultural and Biological Sciences

Horticulture Research Pub Date : 2024-10-23 DOI:10.1093/hr/uhae297

Ling Wang, Mengyu Zhao, Xue Zhang, Ting Zhao, Congbo Huang, Yujin Tang, Yan Li, Chaohong Zhang

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引用次数: 0

Abstract

Cold stress seriously affects the plant growth and development. The ubiquitination system plays an important role by degrading and modifying substrates at the protein level. In this study, the U-box type ubiquitin ligase VviPUB19 gene was induced by low temperature (4°C) in grapevine. In Arabidopsis thaliana, the pub19 mutant, a homologous mutation of VviPUB19, exhibited enhanced cold tolerance, and the resistance phenotype of the mutant could be attenuated by VviPUB19. VviPUB19 overexpressing grape lines exhibited lower cold tolerance. Furthermore, it was revealed that VviPUB19 interacted with the cold-related transcription factors VviICE1, 2 and 3 and VviCBF1 and 2, and was involved in the degradation of them. This is the first time that an E3 ligase (VviPUB19) that interacts with CBFs and affects its protein stability has been identified. It was also shown that VviICE1, 2 and 3 positively regulated VviPUB19 promoter activity. Therefore, our results suggest that VviPUB19 reduces grape cold tolerance via participating in the CBF-dependent pathway.

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泛素连接酶 VviPUB19 通过影响 ICEs 和 CBFs 的稳定性来负向调节葡萄的耐寒性

冷胁迫严重影响植物的生长和发育。泛素化系统通过在蛋白质水平上降解和修饰底物发挥着重要作用。本研究通过低温（4°C）诱导葡萄中的 U-box 型泛素连接酶 VviPUB19 基因。在拟南芥中，VviPUB19的同源突变体pub19突变体表现出更强的耐寒性，VviPUB19可减轻突变体的抗性表型。过表达 VviPUB19 的葡萄品系表现出较低的耐寒性。此外，研究还发现VviPUB19与冷相关转录因子VviICE1、2和3以及VviCBF1和2相互作用，并参与它们的降解。这是首次发现与 CBFs 相互作用并影响其蛋白质稳定性的 E3 连接酶（VviPUB19）。研究还表明，VviICE1、2 和 3 能正向调节 VviPUB19 启动子的活性。因此，我们的研究结果表明，VviPUB19 通过参与 CBF 依赖性途径来降低葡萄的耐寒性。

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来源期刊

Horticulture Research Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

11.20

自引率

6.90%

发文量

367

审稿时长

20 weeks

期刊介绍： Horticulture Research, an open access journal affiliated with Nanjing Agricultural University, has achieved the prestigious ranking of number one in the Horticulture category of the Journal Citation Reports ™ from Clarivate, 2022. As a leading publication in the field, the journal is dedicated to disseminating original research articles, comprehensive reviews, insightful perspectives, thought-provoking comments, and valuable correspondence articles and letters to the editor. Its scope encompasses all vital aspects of horticultural plants and disciplines, such as biotechnology, breeding, cellular and molecular biology, evolution, genetics, inter-species interactions, physiology, and the origination and domestication of crops.