Sensitive detection of α-amylase based on host-guest inclusion system of γ-cyclodextrin and dansyl-derived diphenylalanine.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Pub Date : 2025-02-05 Epub Date: 2024-10-16 DOI:10.1016/j.saa.2024.125291

Yu Liu, Yutian Jiao, Longjun Xiong, Gongli Wei, Baocai Xu, Guiju Zhang, Ce Wang, Li Zhao

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Abstract

A highly sensitive detection system for α-amylase was developed via host-guest complexation between γ-cyclodextrin and dansyl-modified diphenylalanine (FF-Dns). The host-guest inclusion of FF-Dns into the cavity of γ-CD in a HEPES buffer solution (10 mM, pH 7.4) significantly enhanced the fluorescence intensity, and the emission wavelength gradually shifted from 558 to 535 nm. The hydrolysis of γ-CD by the addition of α-amylase released FF-Dns, leading to the recovery of the fluorescence emission characteristics. Therefore, the FF-Dns/γ-CD host-guest complexation system can serve as a platform for the sensitive detection of α-amylase with good selectivity against potential interference. The limit of detection (LOD) of the system was 0.004 U/mL, with a linear working range of 0-6 U/mL. The detection assay was successfully applied in 0.1 % serum, achieving an LOD of 0.017 U/mL and a linear working range of 0-10 U/mL.

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基于γ-环糊精和丹酰衍生二苯丙氨酸的主-客包合系统的α-淀粉酶灵敏检测。

通过γ-环糊精和丹酰改性二苯基丙氨酸（FF-Dns）之间的主-客复合物，开发了一种高灵敏度的α-淀粉酶检测系统。在 HEPES 缓冲溶液（10 mM，pH 7.4）中，将 FF-Dns 加入到 γ-CD 的空腔中，可显著增强荧光强度，发射波长从 558 nm 逐渐转移到 535 nm。加入α-淀粉酶后，γ-CD被水解，释放出FF-Dns，从而恢复了荧光发射特性。因此，FF-Dns/γ-CD 主-客复合物系统可作为灵敏检测α-淀粉酶的平台，对潜在干扰具有良好的选择性。该系统的检测限（LOD）为 0.004 U/mL，线性工作范围为 0-6 U/mL。该检测方法成功地应用于 0.1 % 血清中，检测限为 0.017 U/mL，线性工作范围为 0-10 U/mL。

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Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

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公司名称	产品信息	采购帮参考价格
上海源叶	Bovine serum albumin (BSA)
上海源叶	Trypsin
上海源叶	Pepsin