Evaluation of riboflavin, nanocurcumin, and hydrogen peroxide under light conditions: Reduction of mature dental biofilms and enamel mineral loss

IF 3.1 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy Pub Date : 2024-10-18 DOI:10.1016/j.pdpdt.2024.104379

Shima Afrasiabi , Ahmed Qays Khudhur AL． Gburi , Ladan Ranjbar Omrani , Nasim Chiniforush , Zohreh Moradi

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引用次数: 0

Abstract

Background

Biofilms are a potential harbor for many microorganisms. The aim of this study was to test the efficacy of riboflavin (Rib), nano-micelle curcumin (NC), and hydrogen peroxide (HP), alone and in combination with the respective light (light-emitting diode (LED) or 980 nm diode laser) on the reduction of Streptococcus mutans and Lactobacillus acidophilus dual-species biofilms and their effect on the enamel mineral loss.

Materials and methods

The biofilms were formed on saliva-coated enamel slabs. Then, the biofilms were treated with antimicrobial photodynamic therapy (PDT) based on LED, Rib, and NC photosensitizers and with HP also based on a 980 nm diode laser (n = 8 per group). A crystal violet assay was performed to determine the reduction of the dual-species biofilms. The enamel slabs were analyzed for calcium and phosphorus content by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX).

Results

While HP-PDT showed a reduction of 37% (p < 0.001), PDT with NC resulted in an even greater reduction of dual-species biofilms (40%, p < 0.001) than HP- and Rib-mediated PDT. In the EDX test, no significant difference was found between the control group and the treatment groups.

Conclusions

The use of natural photosensitizers such as NC in PDT has an effect that may be potentially important in reducing caries-causing bacteria.

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在光照条件下评估核黄素、纳米姜黄素和过氧化氢：减少成熟牙齿生物膜和釉质矿物质流失。

背景：生物膜是许多微生物的潜在庇护所。本研究的目的是测试核黄素（Rib）、纳米姜黄素（NC）和过氧化氢（HP）单独或与相应的光（发光二极管（LED）或 980 纳米二极管激光）结合使用对减少变异链球菌和嗜酸乳杆菌双种生物膜的功效及其对釉质矿物质流失的影响：在涂有唾液的搪瓷板上形成生物膜。然后，使用基于 LED、Rib 和 NC 光敏剂的抗菌光动力疗法（PDT）以及基于 980 纳米二极管激光的 HP 光动力疗法（每组 8 人）对生物膜进行治疗。采用水晶紫检测法确定双种生物膜的减少情况。通过扫描电子显微镜（SEM）和能量色散 X 射线光谱（EDX）分析珐琅质板块的钙和磷含量：HP-PDT减少了37%的生物膜（p < 0.001），与HP和Rib介导的PDT相比，NC介导的PDT减少了更多的双种生物膜（40%，p < 0.001）。在 EDX 测试中，对照组和治疗组之间没有发现明显差异：结论：在光动力疗法中使用天然光敏剂（如 NC）具有减少致龋细菌的潜在重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Photodiagnosis and Photodynamic Therapy ONCOLOGY-

CiteScore

5.80

自引率

24.20%

发文量

509

审稿时长

50 days

期刊介绍： Photodiagnosis and Photodynamic Therapy is an international journal for the dissemination of scientific knowledge and clinical developments of Photodiagnosis and Photodynamic Therapy in all medical specialties. The journal publishes original articles, review articles, case presentations, "how-to-do-it" articles, Letters to the Editor, short communications and relevant images with short descriptions. All submitted material is subject to a strict peer-review process.