Molecular identification of EhCRT gene Calreticulin isolated from children infected with Entamoeba histolytica.

IF 0.8 4区医学 Q3 MEDICINE, GENERAL & INTERNAL

Journal of the Pakistan Medical Association Pub Date : 2024-10-01 DOI:10.47391/JPMA-BAGH-16-52

Noor Khalf Al-Mashhadani, Hatham Ebraheem Khalil, Ahmed Sattar Abood

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引用次数: 0

Abstract

Objective: To detect the gene of entamoeba histolytica by polymerase chain reaction and investigate the expression of immunogene entamoeba histolytica calreticulin in stool samples of infected patients.

Methods: The case control study was conducted at Central Teaching Hospital of Paediatrics and Al Mahmoudia General Hospital, Iraq, from December 30, 2020, to September 1, 2021, and comprised diarrhoeal faecal samples collected from 86 children with age ranging from ˂1 year to 13 years who were suspected of having been infected with entamoeba histolytica. Microscopically positive samples were then subjected to conventional and real-time polymerase chain reaction for the detection of entamoeba histolytica HM1:IMSS strain using Phage shock protein (Psp) gene sequences and detection of entamoeba histolytica calreticulin expression.

Results: Of the 86 patients, 71(82.6%) were found to be infected with entamoeba histolytica; 39(54.93%) boys and 32(45.07%) girls. The remaining 15(17.4%) patients were taken as non-amoebic controls; 8(53.3%) boys and 7(46.7%) girls. There were 36(50.70%) cases and 8(53.33%) controls aged 1-4 years. Among the Entamoeba histolytica gene was detected in 44(62%) of the cases using conventional polymerase chain reaction, and immunogene entamoeba histolytica calreticulin was expressed in 36(50.7%) using real-time polymerase chain reaction. Data was analysed using SPSS 24.

Conclusions: Polymerase chain reaction was found to be a useful tool for diagnosing entamoeba histolytica infection in children.

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从感染组织溶解恩塔米巴虫的儿童中分离出的 EhCRT 基因 Calreticulin 的分子鉴定。

目的通过聚合酶链反应检测感染者粪便样本中的组织溶解恩塔米巴虫基因，并研究免疫基因组织溶解恩塔米巴虫钙网蛋白的表达：病例对照研究于 2020 年 12 月 30 日至 2021 年 9 月 1 日在伊拉克中央儿科教学医院和 Al Mahmoudia 综合医院进行，包括从 86 名年龄介于 1 岁至 13 岁的疑似感染组织溶解恩塔米巴虫的儿童身上采集的腹泻粪便样本。然后对显微镜下呈阳性的样本进行传统和实时聚合酶链反应，利用噬菌体休克蛋白（Psp）基因序列检测组织溶解恩塔米巴虫 HM1:IMSS 菌株，并检测组织溶解恩塔米巴虫钙网蛋白的表达：在86名患者中，有71人（82.6%）被发现感染了组织溶解恩塔米巴虫，其中39人（54.93%）为男孩，32人（45.07%）为女孩。其余 15 名（17.4%）患者为非阿米巴对照组，其中 8 名（53.3%）为男孩，7 名（46.7%）为女孩。年龄在 1-4 岁的病例有 36 例（50.70%），对照组有 8 例（53.33%）。其中，44 例（62%）病例使用传统聚合酶链式反应检测到了组织溶解恩塔米巴虫基因，36 例（50.7%）病例使用实时聚合酶链式反应检测到了免疫基因组织溶解恩塔米巴虫钙网蛋白。数据分析采用 SPSS 24.Conclusions：聚合酶链反应被认为是诊断儿童组织溶解埃塔米巴感染的有效工具。

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来源期刊

Journal of the Pakistan Medical Association Medicine-General Medicine

CiteScore

1.10

自引率

11.10%

发文量

983

审稿时长

3-6 weeks

期刊介绍： Primarily being a medical journal, JPMA publishes scholarly research focusing on the various fields in the areas of health and medical education. It publishes original research describing recent advances in health particularly clinical studies, clinical trials, assessments of pathogens of diagnostic importance, medical genetics and epidemiological studies. Review articles highlighting importance of various issues in the domain of public health, drug research and medical education are also accepted. As a leading journal of South Asia, JPMA remains cognizant of the recent advances in the rapidly growing fields of biomedical sciences, it invites and encourages scholars to write short reviews and invited editorials on the emerging issues. We particularly aim to promote health standards of developing countries by encouraging manuscript submissions on issues affecting the public health and health delivery services.