Histological assessment of the effect of 50mW red laser on retina at variable time intervals: animal model.

IF 0.8 4区医学 Q3 MEDICINE, GENERAL & INTERNAL

Journal of the Pakistan Medical Association Pub Date : 2024-10-01 DOI:10.47391/JPMA-BAGH-16-93

Qamar Aqeel Shams, Ameen Mohammad Alsudani, Mustafa Salih Almusawi

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引用次数: 0

Abstract

Objective: To assess the histopathological effect of 50milliWatt red laser pointer on mice retinal tissue at variable exposure times.

Methods: The experimental study was conducted in the Postgraduate Laboratory of the Unit of Medical Physics, Department of Physiology, College of Medicine, Mustansiriyah University, Baghdad, Iraq, from November 2020 to April 2021, and comprised albino mice. Histopathological effects on the retinal tissues were evaluated microscopically using conventional haematoxylin and eosin stain just after being exposed to commercially available 50 milliWatt red laser pointers with 630nm wavelength at 1, 2 and 3 minutes, grouped as R1, R2 and R3, respectively. The laser beam was focussed on the right eye of the mice sedated with sodium pentobarbital, reserving the left eye for use as a control group. Data was analysed using SPSS 24.

Results: Of the 12 mice, 4(33.3%) were in each of the 3 groups. There was significantly destructive effect of red laser on retinal outer cellular layers in all the groups compared to the controls (p<0.05). The effect was significant between R1 and R2 (p=0.0001), and between R2 and R3 (p=0.02).

Conclusions: The handheld commercial red laser pointers could harmfully affect a wide range of mice retina and choroid.

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50mW 红色激光对视网膜不同时间间隔影响的组织学评估：动物模型。

目的评估不同照射时间下 50 毫瓦红色激光笔对小鼠视网膜组织的组织病理学影响：实验研究于 2020 年 11 月至 2021 年 4 月在伊拉克巴格达 Mustansiriyah 大学医学院生理学系医学物理学组研究生实验室进行，实验对象为白化小鼠。小鼠刚接触市售波长为 630nm 的 50 毫瓦红色激光指示器 1、2 和 3 分钟（分别为 R1、R2 和 R3）后，使用传统的血红素和伊红染色法在显微镜下评估视网膜组织的组织病理学影响。激光束对准用戊巴比妥钠镇静的小鼠右眼，保留左眼作为对照组。数据使用 SPSS 24 进行分析：在 12 只小鼠中，3 组各有 4 只（33.3%）。与对照组相比，所有组的红色激光对视网膜外细胞层都有明显的破坏作用（p结论：手持式商用红色激光指示器会对小鼠视网膜和脉络膜造成大范围的伤害。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Pakistan Medical Association Medicine-General Medicine

CiteScore

1.10

自引率

11.10%

发文量

983

审稿时长

3-6 weeks

期刊介绍： Primarily being a medical journal, JPMA publishes scholarly research focusing on the various fields in the areas of health and medical education. It publishes original research describing recent advances in health particularly clinical studies, clinical trials, assessments of pathogens of diagnostic importance, medical genetics and epidemiological studies. Review articles highlighting importance of various issues in the domain of public health, drug research and medical education are also accepted. As a leading journal of South Asia, JPMA remains cognizant of the recent advances in the rapidly growing fields of biomedical sciences, it invites and encourages scholars to write short reviews and invited editorials on the emerging issues. We particularly aim to promote health standards of developing countries by encouraging manuscript submissions on issues affecting the public health and health delivery services.