The usefulness of evaluating PTEN expression for accurate grading of phyllodes tumors

IF 1.5 4区医学 Q3 PATHOLOGY

Annals of Diagnostic Pathology Pub Date : 2024-10-16 DOI:10.1016/j.anndiagpath.2024.152384

Donghyun Kim , Kwang-Sun Suh , Mee-Ran Kim , Song-Yi Choi

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引用次数: 0

Abstract

Phyllodes tumors (PTs) are classified as benign, borderline, or malignant based on histologic characteristics. However, because histological criteria are subjective and diagnosis requires integrating multiple findings, discrepancies often occur between observers. Therefore, it is necessary to discover biomarkers based on the molecular characteristics of PTs. This study aimed to identify dysregulated microRNAs (miRNAs) in PTs through miRNA profiling and determine whether expression of their target genes could be useful as PT biomarkers. MiRNA profiling was performed on 13 PTs (three malignant, three borderline, seven benign) and six fibroadenomas, and predicted target genes of dysregulated miRNAs were selected using three computation algorithms. The expression of two miRNAs, miR-155 and miR-200c, was 1.69-fold and 1.61-fold higher, respectively, in borderline and malignant PT groups than in the benign PT group (p < 0.05). KEGG pathway analysis revealed that the 374 target genes of these two miRNAs (miR-155 and miR200c) participated in several signaling pathways, adherens junction, cell cycle, and pathway in cancer. Immunohistochemical staining for PTEN, one of candidate target genes of miR-200c, was performed on whole slides of PT tissue classified as malignant (n = 9), borderline (n = 12), or benign (n = 21). Stromal tumor cells of high-grade PTs (borderline and malignant) had significantly lower PTEN expression than those of low-grade PTs (benign) (p-value ≤0.001). Semiquantitative analysis of PTEN expression, score 0–8, revealed that it correlated with histologic findings. Low PTEN expression (s-score of 6 or less) was used as a diagnostic criterion for high-grade PTs, it showed 100 % sensitivity and 95.2 % specificity in 42 cases of PTs. Currently, PT grading based solely on subjective histologic findings is challenging, but semiquantitative PTEN expression analysis could provide a more accurate and objective way to grade PTs.

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评估 PTEN 表达对植物瘤准确分级的作用。

皮样瘤（PT）根据组织学特征分为良性、边缘性和恶性。然而，由于组织学标准是主观的，而且诊断需要综合多种发现，因此观察者之间经常会出现分歧。因此，有必要根据 PT 的分子特征发现生物标志物。本研究旨在通过miRNA图谱分析鉴定PT中失调的microRNA（miRNA），并确定其靶基因的表达是否可作为PT的生物标志物。研究人员对13例PT（3例恶性、3例边缘性、7例良性）和6例纤维腺瘤进行了miRNA图谱分析，并利用三种计算算法筛选出调控失调miRNA的预测靶基因。miR-155和miR-200c这两种miRNA在边缘型和恶性PT组的表达量分别是良性PT组的1.69倍和1.61倍（P＜0.05）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Diagnostic Pathology 医学-病理学

CiteScore

3.90

自引率

5.00%

发文量

149

审稿时长

26 days

期刊介绍： A peer-reviewed journal devoted to the publication of articles dealing with traditional morphologic studies using standard diagnostic techniques and stressing clinicopathological correlations and scientific observation of relevance to the daily practice of pathology. Special features include pathologic-radiologic correlations and pathologic-cytologic correlations.