Prognostic value of miR-190a-5p in renal cell cancer and its regulatory effect on tumor progression.

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Journal of Biological Markers Pub Date : 2024-10-17 DOI:10.1177/03936155241290251

Jun Liu, Lili Zhang, Zhancheng Wang, Hu Li, Bo Wang, Xiaoqiang Liu

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引用次数: 0

Abstract

Purpose: As a usual malignant tumor in urinary system, renal cell cancer is regulated by microRNAs (miRNAs). This study revealed the prognostic value and regulatory effect of miR-190a-5p in renal cell cancer patients.

Methods: A total of 253 renal cell cancer patients were included for prognostic value analysis. The target gene of miR-190a-5p was detected by luciferase reporter assay. Cell Counting Kit-8 analysis and Transwell analysis were performed to explore the proliferation, removal capability, and invasiveness of 786-0 and A498 cells. Prognostic value was calculated by Kaplan-Meier curve and Cox regression analysis.

Results: miR-190a-5p was more down-regulated in tumor tissues than in adjacent tissues. Renal cell cancer cases were differed as low and high groups ground on mean miR-190a-5p expression in tumor tissues. Overall survival probability was obviously high in patients with high miR-190a-5p level (log-rank test P = 0.011). Cox regression analysis revealed that miR-190a-5p expression (relative risk (RR) = 1.751, 95% confidence interval (CI) = 1.057-2.900, P = 0.030) and tumor node metastasis stage (RR = 1.719, 95% CI = 1.059-2.792, P = 0.028) were specialty indicators for poor renal cell cancer prognosis. GDF11 was directly targeting miR-190a-5p. Overexpressed miR-190a-5p could reduce the GDF11 expression, proliferation, removal capability, and invasiveness of renal cell cancer 786-0 and A498 cells. Elevated GDF11 could lead to a changeover of proliferation, removal capability, and invasiveness inhibition, which is induced by miR-190a-5p.

Conclusion: miR-190a-5p was reduced in renal cell cancer tissues, and predicted worse outcomes of renal cell cancer cases. Overexpressed miR-190a-5p could restrain the proliferation, removal capability, and invasiveness of renal cell cancer cells via suppressing GDF11.

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miR-190a-5p 在肾细胞癌中的预后价值及其对肿瘤进展的调控作用

目的：作为泌尿系统常见的恶性肿瘤，肾细胞癌受到微RNA（miRNA）的调控。本研究揭示了 miR-190a-5p 在肾细胞癌患者中的预后价值和调控作用：方法：共纳入 253 例肾癌患者进行预后价值分析。通过荧光素酶报告实验检测 miR-190a-5p 的靶基因。细胞计数试剂盒-8分析和Transwell分析用于探讨786-0和A498细胞的增殖、清除能力和侵袭性。结果显示：miR-190a-5p在肿瘤组织中的下调程度高于邻近组织。根据肿瘤组织中 miR-190a-5p 的平均表达量，将肾细胞癌病例分为低组和高组。miR-190a-5p水平高的患者总生存概率明显较高（log-rank检验 P = 0.011）。Cox回归分析显示，miR-190a-5p表达（相对风险（RR）= 1.751，95%置信区间（CI）= 1.057-2.900，P = 0.030）和肿瘤结节转移分期（RR = 1.719，95% CI = 1.059-2.792，P = 0.028）是肾细胞癌不良预后的特异性指标。GDF11直接靶向miR-190a-5p。过表达的miR-190a-5p可降低肾细胞癌786-0和A498细胞的GDF11表达、增殖、清除能力和侵袭性。结论：miR-190a-5p在肾细胞癌组织中的表达量减少，预示着肾细胞癌患者的预后较差。过表达的 miR-190a-5p 可通过抑制 GDF11 抑制肾细胞癌细胞的增殖、清除能力和侵袭性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Biological Markers 医学-生物工程与应用微生物

CiteScore

4.10

自引率

0.00%

发文量

期刊介绍： IJBM is an international, online only, peer-reviewed Journal, which publishes original research and critical reviews primarily focused on cancer biomarkers. IJBM targets advanced topics regarding the application of biomarkers in oncology and is dedicated to solid tumors in adult subjects. The clinical scenarios of interests are screening and early diagnosis of cancer, prognostic assessment, prediction of the response to and monitoring of treatment.