CircLPHN3 correlates with prognosis in colorectal cancer and regulates cellular processes by targeting miR-142-5p.

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

International Journal of Biological Markers Pub Date : 2024-10-18 DOI:10.1177/03936155241287219

JiWen Zhang, Yan Cai

{"title":"CircLPHN3 correlates with prognosis in colorectal cancer and regulates cellular processes by targeting miR-142-5p.","authors":"JiWen Zhang, Yan Cai","doi":"10.1177/03936155241287219","DOIUrl":null,"url":null,"abstract":"Background: Colorectal cancer (CRC) is often diagnosed late and has a poor prognosis. Circular RNAs (circRNAs) have been identified as prognostic biomarkers in various cancers, including CRC.Objective: The objective was to elucidate the role of circLPHN3 (hsa_circ_0069865) in CRC progression and to provide a promising prognostic marker for CRC.Methods: CircLPHN3 was identified through bioinformatics analysis of the GSE121842 dataset. The levels of circLPHN3 in CRC samples were analyzed by real time-quantitative polymerase chain reaction. Its clinical significance was assessed using the Kaplan-Meier curve and multivariate Cox regression. Downstream microRNAs of circLPHN3 were predicted with the RNAhybrid, Circular RNA Interactome, and starBase online databases. The target of miR-142-5p was predicted using miRDB, TargetScanHuman, starBase, and miRWalk databases. The relationship between circLPHN3, miR-142-5p, and LDB2 was verified by dual luciferase reporter assay. The function of circLPHN3 on CRC cell growth and metastasis was measured using Transwell and the cell counting kit-8 assay.Results: Significant downregulation of circLPHN3 was found in CRC. CircLPHN3 was closely related to higher tumor node metastasis stage, lymph node metastasis, and predicted unfavorable prognosis. miR-142-5p was highly expressed in CRC and its expression was negatively regulated by circLPHN3. Overexpression of circLPHN3 curbed CRC cell growth, migration, and invasion, mediated by miR-142-5p. Moreover, LDB2 was identified as a target of miR-142-5p.Conclusion: CircLPHN3 acted as a prognostic biomarker and tumor suppressor for CRC via modulating miR-142-5p.","PeriodicalId":50334,"journal":{"name":"International Journal of Biological Markers","volume":" ","pages":"3936155241287219"},"PeriodicalIF":2.3000,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biological Markers","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1177/03936155241287219","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Colorectal cancer (CRC) is often diagnosed late and has a poor prognosis. Circular RNAs (circRNAs) have been identified as prognostic biomarkers in various cancers, including CRC.

Objective: The objective was to elucidate the role of circLPHN3 (hsa_circ_0069865) in CRC progression and to provide a promising prognostic marker for CRC.

Methods: CircLPHN3 was identified through bioinformatics analysis of the GSE121842 dataset. The levels of circLPHN3 in CRC samples were analyzed by real time-quantitative polymerase chain reaction. Its clinical significance was assessed using the Kaplan-Meier curve and multivariate Cox regression. Downstream microRNAs of circLPHN3 were predicted with the RNAhybrid, Circular RNA Interactome, and starBase online databases. The target of miR-142-5p was predicted using miRDB, TargetScanHuman, starBase, and miRWalk databases. The relationship between circLPHN3, miR-142-5p, and LDB2 was verified by dual luciferase reporter assay. The function of circLPHN3 on CRC cell growth and metastasis was measured using Transwell and the cell counting kit-8 assay.

Results: Significant downregulation of circLPHN3 was found in CRC. CircLPHN3 was closely related to higher tumor node metastasis stage, lymph node metastasis, and predicted unfavorable prognosis. miR-142-5p was highly expressed in CRC and its expression was negatively regulated by circLPHN3. Overexpression of circLPHN3 curbed CRC cell growth, migration, and invasion, mediated by miR-142-5p. Moreover, LDB2 was identified as a target of miR-142-5p.

Conclusion: CircLPHN3 acted as a prognostic biomarker and tumor suppressor for CRC via modulating miR-142-5p.

查看原文本刊更多论文

CircLPHN3 与结直肠癌的预后有关，并通过靶向 miR-142-5p 调节细胞过程。

背景：大肠癌（CRC）通常诊断较晚，预后较差。环状 RNA（circRNA）已被确定为包括 CRC 在内的多种癌症的预后生物标志物：目的：阐明circLPHN3（hsa_circ_0069865）在CRC进展中的作用，并为CRC提供一个有希望的预后标志物：方法：通过对 GSE121842 数据集进行生物信息学分析，确定了 circLPHN3。通过实时定量聚合酶链反应分析了 CRC 样本中 circLPHN3 的水平。采用 Kaplan-Meier 曲线和多变量 Cox 回归评估了其临床意义。利用 RNAhybrid、Circular RNA Interactome 和 starBase 在线数据库预测了 circLPHN3 的下游微RNA。利用 miRDB、TargetScanHuman、starBase 和 miRWalk 数据库预测了 miR-142-5p 的靶点。通过双荧光素酶报告实验验证了 circLPHN3、miR-142-5p 和 LDB2 之间的关系。利用Transwell和细胞计数试剂盒-8测定了circLPHN3对CRC细胞生长和转移的作用：结果：在 CRC 中发现 circLPHN3 明显下调。miR-142-5p在CRC中高表达，其表达受circLPHN3的负调控。在miR-142-5p的介导下，circLPHN3的过表达抑制了CRC细胞的生长、迁移和侵袭。此外，LDB2被确定为miR-142-5p的靶标：CircLPHN3通过调节miR-142-5p成为CRC的预后生物标志物和肿瘤抑制因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

International Journal of Biological Markers 医学-生物工程与应用微生物

CiteScore

4.10

自引率

0.00%

发文量

期刊介绍： IJBM is an international, online only, peer-reviewed Journal, which publishes original research and critical reviews primarily focused on cancer biomarkers. IJBM targets advanced topics regarding the application of biomarkers in oncology and is dedicated to solid tumors in adult subjects. The clinical scenarios of interests are screening and early diagnosis of cancer, prognostic assessment, prediction of the response to and monitoring of treatment.