Immunohistochemical Expression of MDM2, Bcl-2, SATB2 and Ki-67 in Histological Variants of Unicystic Ameloblastoma.

IF 3.2 Q2 PATHOLOGY
Koustubh Amol Surana, Deepak Pandiar, Reshma Poothakulath Krishnan
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引用次数: 0

Abstract

Aim: To characterize the immunohistochemical expression of MDM2, Bcl-2, SATB2 and Ki-67 in histological variants of unicystic ameloblastoma (UA).

Methodology: Following the ethical approval, forty (40) patients with unicystic ameloblastoma were retrieved from the archives and subjected to immunohistochemistry (IHC). Sociodemographic and clinical data were also retrieved. The results were entered into a Microsoft Excel spreadsheet and analyzed using SPSS software.

Results: Human tooth germs, which served as the control, showed moderate expression of Bcl-2 and MDM2 with slight proliferative activity in ameloblasts and moderate expression of SATB2 in ectomesenchyme and odontoblasts. Luminal UA (Type 1) showed low Ki-67 index and negative to mild Bcl-2 and MDM2 expression, whilst Type 1.2 (luminal and intraluminal), Type 1.2.3 (luminal, intraluminal and mural), and Type 1.3 (luminal and mural), including the recurrent cases, showed moderate to intense expression with high mean Ki-67 index. The difference between the study groups was statistically significant (p value < 0.001). No expression of SATB2 was noted in any histological variant of UA. Furthermore, no significant differences were noted in age, gender, site and location between the groups.

Conclusion: In contrast to luminal variant of UA, mural±intraluminal variants and recurrent cases demonstrate higher expression of Bcl-2 and MDM2 with higher mean Ki-67 index. It may thus be prudent to provide aggressive treatment for cases, not just with mural follicles but also for the patients with intraluminal plexiform proliferation, to prevent recurrence and improve patient outcomes.

MDM2、Bcl-2、SATB2 和 Ki-67 在单囊性母细胞瘤组织学变异中的免疫组化表达
目的:分析单囊性母细胞瘤(UA)组织学变异中MDM2、Bcl-2、SATB2和Ki-67的免疫组化表达特征:在获得伦理批准后,从档案中提取了四十(40)名单囊性母细胞瘤患者,并对其进行了免疫组化(IHC)检查。同时还检索了社会人口学和临床数据。结果输入 Microsoft Excel 电子表格,并使用 SPSS 软件进行分析:结果:作为对照的人类牙胚显示 Bcl-2 和 MDM2 中度表达,在成骨细胞中有轻微的增殖活性,SATB2 在外胚层和牙本质中有中度表达。腔隙性 UA(1 型)的 Ki-67 指数较低,Bcl-2 和 MDM2 表达为阴性至轻度,而 1.2 型(腔隙性和腔内性)、1.2.3 型(腔隙性、腔内性和壁性)和 1.3 型(腔隙性和壁性)(包括复发性病例)则表现为中度至高度表达,平均 Ki-67 指数较高。各研究组之间的差异具有统计学意义(P 值 结论):与腔内变异型 UA 相比,壁内变异型和复发病例的 Bcl-2 和 MDM2 表达较高,平均 Ki-67 指数也较高。因此,为防止复发和改善患者预后,不仅要对壁状滤泡病例,还要对腔内丛状增生的患者进行积极治疗。
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来源期刊
CiteScore
5.70
自引率
9.50%
发文量
99
期刊介绍: Head & Neck Pathology presents scholarly papers, reviews and symposia that cover the spectrum of human surgical pathology within the anatomic zones of the oral cavity, sinonasal tract, larynx, hypopharynx, salivary gland, ear and temporal bone, and neck. The journal publishes rapid developments in new diagnostic criteria, intraoperative consultation, immunohistochemical studies, molecular techniques, genetic analyses, diagnostic aids, experimental pathology, cytology, radiographic imaging, and application of uniform terminology to allow practitioners to continue to maintain and expand their knowledge in the subspecialty of head and neck pathology. Coverage of practical application to daily clinical practice is supported with proceedings and symposia from international societies and academies devoted to this field. Single-blind peer review The journal follows a single-blind review procedure, where the reviewers are aware of the names and affiliations of the authors, but the reviewer reports provided to authors are anonymous. Single-blind peer review is the traditional model of peer review that many reviewers are comfortable with, and it facilitates a dispassionate critique of a manuscript.
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