[Effects of mild moxibustion on visceral hypersensitivity via SCF/c-kit signaling pathway in diarrhea-predominant irritable bowel syndrome rats with syndrome of liver-qi stagnation and spleen deficiency].

Q3 Medicine
Ying-Long Chen, Xue-Mei Shang, Hua-Mei Yu, Zhou Zhu, Ling-Min Jin, Pan-Bi Chen, Yong-Jun Lu, Rong Wang, Xiao-Fang Yang
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引用次数: 0

Abstract

Objectives: To observe the effect of mild moxibustion on stem cell factor (SCF)/tyrosine kinase receptor (c-kit) signaling pathway and visceral hypersensitivity of diarrhea predominant irritable bowel syndrome (IBS-D) model rats with liver-qi stagnation and spleen deficiency syndrome, so as to explore its mechanisms underlying improvement of IBS-D.

Methods: A total of 24 male Wistar rats were randomly divided into normal, IBS-D model, medication and mild moxibustion groups, with 6 rats in each group. The IBS-D model was established by glacial acetic acid (4%) enema plus restraint stress stimulation once daily for 14 days. Rats of the medication group were treated by gavage of pivamium bromide (15 mg/kg) once a day for 14 days. Mild moxibustion was applied to bilateral "Tianshu"(ST25), "Shangjuxu"(ST37) and "Taichong"(LR3) for 20 min, once daily for 14 consecutive days. After the intervention, the rats' general state of each group were observed. The rate of loose stools (LSR), and the minimum volume threshold for abdominal withdrawal reflex(AWR) were observed, and the open field test was used to assess the state of rats' motor activities (including rearing times, grooming times and total number of square-crossings in 5 min). Morphological changes of the colon tissue were observed by hematoxylin-eosin (H.E.) staining, The count of mast cells (MC) in the colon tissues was determined by toluidine blue staining. Contents of serum 5-hydroxytryptamine (5-HT) and substance P (SP) were determined by enzyme-linked immunosorbent assay (ELISA). The relative expression levels of SCF and c-kit mRNAs and proteins in the colon tissues were detected by real-time quantitative PCR and Western blot, respectively.

Results: Compared with the normal group, the body weight, minimum volume threshold of AWR, total numbers of square-crossing, rearing times and grooming times were significantly decreased (P<0.05), and the LSR, number of MC, contents of 5-HT and SP, and the expression levels of SCF and c-kit mRNAs and proteins were considerably increased in the model group (P<0.01). In comparison with the model group after interventions, the body weight, minimum volume threshold of AWR, total numbers of square-crossing, rearing times and grooming times were apparently increased in both medication and moxibustion groups (P<0.05, P<0.01), and the LSR, number of MC, 5-HT and SP contents in both medication and moxibustion groups, and the expression levels of SCF and c-kit mRNA and protein in the moxibustion group (not in the medication group) were obviously decreased (P<0.05, P<0.01). H.E. staining showed that in the model group, a small amount of inflammatory cells in the mucosal layer of colon tissue could be seen. in the medication group, a small number of lymphocytes in colon tissue were observed, while in the mild moxibustion group, a small amount of neutrophils in colon tissue were observed.

Conclusions: Mild moxibustion can reduce visceral hypersensitivity and improve abdominal pain, diarrhea and locomotion state in IBS-D rats with liver-qi stagnation and spleen deficiency syndrome, which may be associated with its functions in reducing the number of MC and the levels of 5-HT and SP and down-regulating the activities of SCF/c-kit signaling pathway.

[温和灸通过 SCF/c-kit 信号通路对以腹泻为主的肝气郁结脾虚型肠易激综合征大鼠内脏超敏反应的影响]
研究目的观察温和灸对肝气郁结脾虚型以腹泻为主的肠易激综合征(IBS-D)模型大鼠干细胞因子(SCF)/酪氨酸激酶受体(c-kit)信号通路及内脏超敏反应的影响,探讨其改善IBS-D的机制:方法:将24只雄性Wistar大鼠随机分为正常组、IBS-D模型组、药物组和温和灸组,每组6只。IBS-D 模型通过冰醋酸(4%)灌肠和束缚应激刺激建立,每天一次,连续 14 天。药物组大鼠通过灌胃匹伐溴胺(15 毫克/千克)进行治疗,每天一次,连续 14 天。对双侧 "天枢"(ST25)、"上枢"(ST37)和 "太冲"(LR3)进行温和艾灸,每次 20 分钟,每天一次,连续 14 天。干预结束后,观察各组大鼠的一般状态。观察大鼠的稀便率(LSR)和腹部退缩反射(AWR)的最小量阈值,并用空场试验评估大鼠的运动活动状态(包括饲养时间、梳理时间和 5 分钟内穿越方阵的总次数)。用苏木精-伊红(H.E.)染色法观察大鼠结肠组织的形态变化,用甲苯胺蓝染色法测定大鼠结肠组织中肥大细胞(MC)的数量。血清中 5-羟色胺(5-HT)和 P 物质(SP)的含量采用酶联免疫吸附法(ELISA)测定。实时定量 PCR 和 Western 印迹分别检测结肠组织中 SCF 和 c-kit mRNA 及蛋白的相对表达水平:结果:与正常组相比,大鼠的体重、AWR最小量阈值、方格交叉总数、饲养次数和梳理次数均显著下降(PPPPPPC结论:温和灸可降低大鼠的体重、AWR最小量阈值、方格交叉总数、饲养次数和梳理次数:温和灸能减轻肝气郁结脾虚型IBS-D大鼠的内脏超敏反应,改善腹痛、腹泻和运动状态,这可能与温和灸能减少MC数量、降低5-羟色胺和SP水平、下调SCF/c-kit信号通路活性有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
针刺研究
针刺研究 Medicine-Medicine (all)
CiteScore
1.30
自引率
0.00%
发文量
0
期刊介绍: Acupuncture Research was founded in 1976. It is an acupuncture academic journal supervised by the State Administration of Traditional Chinese Medicine, co-sponsored by the Institute of Acupuncture of the China Academy of Chinese Medical Sciences and the Chinese Acupuncture Association. This journal is characterized by "basic experimental research as the main focus, taking into account clinical research and reporting". It is the only journal in my country that focuses on reporting the mechanism of action of acupuncture. The journal has been changed to a monthly journal since 2018, published on the 25th of each month, and printed in full color. The manuscript acceptance rate is about 10%, and provincial and above funded projects account for about 80% of the total published papers, reflecting the latest scientific research results in the acupuncture field and has a high academic level. Main columns: mechanism discussion, clinical research, acupuncture anesthesia, meridians and acupoints, theoretical discussion, ideas and methods, literature research, etc.
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