Multiple bloodmeals enhance dissemination of arboviruses in three medically relevant mosquito genera.

IF 3 2区医学 Q1 PARASITOLOGY

Parasites & Vectors Pub Date : 2024-10-19 DOI:10.1186/s13071-024-06531-y

Zannatul Ferdous, Constentin Dieme, Hannah Sproch, Laura D Kramer, Alexander T Ciota, Doug E Brackney, Philip M Armstrong

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引用次数: 0

Abstract

Background: Mosquitoes in nature may acquire multiple bloodmeals (BMs) over the course of their lifetime; however, incorporation of frequent feeding behavior in laboratory vector competence studies is rarely done. We have previously shown that acquisition of a second non-infectious BM can enhance early dissemination of Zika virus (ZIKV), dengue virus, and chikungunya virus in Aedes aegypti and ZIKV in Aedes albopictus mosquitoes, yet it is unknown if other taxonomically-diverse virus-vector pairings show a similar trend under a sequential feeding regimen.

Methods: To test this, we evaluated the impact of a second noninfectious BM on the vector competence of Aedes aegypti and Anopheles quadrimaculatus for Mayaro virus, Culex quinquefasciatus for West Nile virus, Aedes triseriatus for La Crosse virus, and Aedes aegypti for Oropouche virus (OROV). Female mosquitoes were fed BMs containing these viruses and half of them were given a second noninfectious BM at 3 or 4-days post infection. Mosquitoes were harvested at various time points and assayed for virus infection in bodies and disseminated infection in legs by performing reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays.

Results: We found that a second noninfectious BM had no impact on midgut infection rates but increased virus dissemination for all but one of the virus-vector pairings- Ae. aegypti and OROV. Unlike the other arboviruses under consideration, which are strictly mosquito-borne, biting midges (Culicoides spp.) serve as the main vector of OROV and this virus rarely disseminated to the mosquito leg tissue in our study.

Conclusions: Taken together, our findings show that sequential blood feeding enhances virus dissemination across diverse arbovirus-vector pairings, representing three mosquito genera and virus families, but a second BM was insufficient to overcome a strong midgut virus escape barrier in a nonnatural virus-vector pairing.

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多次吸血加强了三种医学相关蚊属中虫媒病毒的传播。

背景：自然界中的蚊子在其一生中可能会获得多次血餐（BM）；然而，在实验室病媒能力研究中很少纳入频繁摄食行为。我们之前已经证明，埃及伊蚊获得第二种非感染性BM可增强寨卡病毒（ZIKV）、登革热病毒和基孔肯雅病毒的早期传播，白纹伊蚊获得ZIKV的早期传播：为了验证这一点，我们评估了第二个非感染性 BM 对埃及伊蚊和四按蚊（Anopheles quadrimaculatus）感染 Mayaro 病毒、库蚊（Culex quinquefasciatus）感染西尼罗河病毒、三色伊蚊（Aedes triseriatus）感染 La Crosse 病毒以及埃及伊蚊（Aedes aegypti）感染 Oropouche 病毒（OROV）的病媒能力的影响。给雌蚊喂食含有这些病毒的基础代谢物，并在感染后 3 或 4 天给其中一半的蚊子喂食第二次非感染性基础代谢物。在不同的时间点收获蚊子，并通过反转录定量聚合酶链反应（RT-qPCR）检测蚊子体内的病毒感染情况和腿部的传播感染情况：结果：我们发现，第二个非感染性 BM 对中肠感染率没有影响，但对所有病毒-载体配对（埃及蚁和 OROV）而言，除一个病毒-载体配对外，病毒传播率都会增加。与其他严格由蚊子传播的虫媒病毒不同，叮咬蠓（Culicoides spp.）是 OROV 的主要传播媒介，在我们的研究中，这种病毒很少传播到蚊子腿部组织：总之，我们的研究结果表明，在代表三个蚊属和病毒科的不同虫媒病毒-病媒配对中，连续采血可增强病毒传播，但在非天然病毒-病媒配对中，第二次采血不足以克服强大的中肠病毒逃逸障碍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Parasites & Vectors 医学-寄生虫学

CiteScore

6.30

自引率

9.40%

发文量

433

审稿时长

1.4 months

期刊介绍： Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish. Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.