Measuring multiple intracellular biochemical properties of proteins with next-generation sequencing

IF 36.1 1区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Nature Methods Pub Date : 2024-10-21 DOI:10.1038/s41592-024-02461-w

引用次数: 0

Abstract

We developed LABEL-seq, a platform that enables measurement of protein properties and functions at scale by leveraging the intracellular self-assembly of an RNA-binding domain (RBD) and protein-encoding RNA barcode. Enrichment of RBD–protein fusions, followed by high-throughput sequencing of the co-enriched barcodes, enables the profiling of protein abundance, activity, interactions and druggability at scale.

Abstract Image

查看原文本刊更多论文

利用新一代测序技术测量蛋白质的多种细胞内生化特性。

我们开发了 LABEL-seq，这是一个利用 RNA 结合域（RBD）和蛋白质编码 RNA 条形码的细胞内自组装来大规模测量蛋白质特性和功能的平台。通过富集 RBD 蛋白融合体，然后对共同富集的条形码进行高通量测序，就能大规模分析蛋白质的丰度、活性、相互作用和可药性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Nature Methods 生物-生化研究方法

CiteScore

58.70

自引率

1.70%

发文量

326

审稿时长

1 months

期刊介绍： Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.