Enterobacterales Producing ESBLs and AmpC in Fresh Vegetables from Tebessa City, Algeria.

IF 2.3 4区医学 Q3 INFECTIOUS DISEASES

Microbial drug resistance Pub Date : 2024-10-22 DOI:10.1089/mdr.2024.0042

Amel Amra, Manel Debabza, Raoudha Dziri, Abdelbasset Mechai, Hadda Imene Ouzari, Naouel Klibi

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Abstract

This study aimed to evaluate the contamination levels of fresh products by ESBLs-producing Enterobacterales (ESBLs-E) or AmpC-producing Enterobacterales and characterize ESBLs genes. A total of 132 samples (67 vegetables and 65 fruits) were collected from markets in Tebessa, eastern Algeria. Among the samples, 16 third-generation cephalosporin-resistant Enterobacterales isolates were identified with a prevalence of 19.40% in vegetable samples, while there was no positive finding in fruit samples. Isolates showed resistance to most β-lactams, and all of them displayed multidrug resistance. Phenotypic tests for ESBLs detection, using double-disk synergy test and double-disk test were positive for 14 strains, including Klebsiella pneumoniae (n = 5), Klebsiella oxytoca (n = 4), Klebsiella terrigena (n = 2), Kluyvera spp. (n = 2), and Enterobacter cloacae (n = 1). Two AmpC-producing strains (Citrobacter freundii and E. cloacae) were identified through the AmpC disk test. Contamination rates of vegetables by ESBLs-E and AmpC-producing Enterobacterales were 19.40% and 2.98%, respectively. PCR results showed the presence of at least one ESBL gene in seven selected strains, with the dominance of bla_CTX-M gene. Notably, K. pneumoniae strains showed the co-occurrence of two or three genes. Sequencing identified uncommon variants of ESBLs genes for the first time in Algeria, including bla_CTX-M-79 (2/7), bla_CTX-M-107 (2/7), bla_CTX-M-117 (2/7), bla_TEM-112 (1/7), bla_TEM-125 (2/7), bla_TEM-194 (1/7), and bla_SHV-176 (3/7).

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阿尔及利亚特贝萨市新鲜蔬菜中产生 ESBLs 和 AmpC 的肠杆菌。

本研究旨在评估产 ESBLs 肠杆菌（ESBLs-E）或产 AmpC 肠杆菌对新鲜产品的污染程度，并确定 ESBLs 基因的特征。研究人员从阿尔及利亚东部泰贝萨的市场上共采集了 132 个样本（67 种蔬菜和 65 种水果）。在这些样本中，发现了 16 个对第三代头孢菌素耐药的肠杆菌分离物，其中蔬菜样本的感染率为 19.40%，而水果样本中没有阳性发现。分离菌株对大多数β-内酰胺类药物都有耐药性，而且所有分离菌株都具有多重耐药性。使用双盘协同试验和双盘试验对 14 株菌株进行 ESBLs 检测的表型测试结果呈阳性，其中包括肺炎克雷伯菌（5 株）、土生克雷伯菌（4 株）、特雷根克雷伯菌（2 株）、克鲁维拉属（2 株）和泄殖腔肠杆菌（1 株）。通过 AmpC 盘测试，确定了两株产 AmpC 的菌株（自由柠檬酸杆菌和泄殖腔肠杆菌）。蔬菜中产ESBLs-E和产AmpC肠杆菌的污染率分别为19.40%和2.98%。聚合酶链式反应（PCR）结果表明，7 株被选中的菌株至少含有一种 ESBL 基因，其中以 blaCTX-M 基因为主。值得注意的是，肺炎克氏菌菌株同时存在两种或三种基因。测序首次在阿尔及利亚发现了不常见的 ESBLs 基因变体，包括 blaCTX-M-79（2/7）、blaCTX-M-107（2/7）、blaCTX-M-117（2/7）、blaTEM-112（1/7）、blaTEM-125（2/7）、blaTEM-194（1/7）和 blaSHV-176（3/7）。

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来源期刊

Microbial drug resistance 医学-传染病学

CiteScore

6.00

自引率

3.80%

发文量

118

审稿时长

6-12 weeks

期刊介绍： Microbial Drug Resistance (MDR) is an international, peer-reviewed journal that covers the global spread and threat of multi-drug resistant clones of major pathogens that are widely documented in hospitals and the scientific community. The Journal addresses the serious challenges of trying to decipher the molecular mechanisms of drug resistance. MDR provides a multidisciplinary forum for peer-reviewed original publications as well as topical reviews and special reports. MDR coverage includes: Molecular biology of resistance mechanisms Virulence genes and disease Molecular epidemiology Drug design Infection control.