Performance of 5 commercial ELISA kits for the detection of antibody to bovine leukemia virus.

IF 1.2 3区农林科学 Q3 VETERINARY SCIENCES

Journal of Veterinary Diagnostic Investigation Pub Date : 2024-10-14 DOI:10.1177/10406387241280629

Vesna Milićević, Dimitrije Glišić, Ljubiša Veljović, Zorana Zurovac Sapundžić, Sofija Šolaja, Nemanja Jezdimirović

{"title":"Performance of 5 commercial ELISA kits for the detection of antibody to bovine leukemia virus.","authors":"Vesna Milićević, Dimitrije Glišić, Ljubiša Veljović, Zorana Zurovac Sapundžić, Sofija Šolaja, Nemanja Jezdimirović","doi":"10.1177/10406387241280629","DOIUrl":null,"url":null,"abstract":"<p><p>To prevent significant economic losses, some countries have successfully eradicated enzootic bovine leukosis (EBL), which is caused by bovine leukemia virus (BLV) infection. In Serbia, efforts to eliminate EBL commenced in the late 1990s. Recognizing the disparities in test selection among laboratories and variations in quality, we evaluated the diagnostic sensitivity and specificity of commercial ELISAs using field samples in Serbia. Using 5 commercial ELISA kits, we tested 138 cattle serum samples, submitted for confirmatory testing between 2020 and 2023, along with 100 serum samples from BLV-negative herds. We found 100% agreement of the ID Screen BLV Competition (IDvet), Svanovir BLV gp51-Ab (Svanova), and INgezim BLV Compac 2.0 (Ingenasa) ELISAs. We observed 93% agreement comparing these 3 kits to the Bovine Leukemia Virus Antibody test kit (VMRD). Agreements of 92% and 88.4% were determined between Idexx and IDvet, Svanova, and Ingenasa kits, and between Idexx and VMRD kits, respectively.</p>","PeriodicalId":17579,"journal":{"name":"Journal of Veterinary Diagnostic Investigation","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11559717/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Diagnostic Investigation","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1177/10406387241280629","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

To prevent significant economic losses, some countries have successfully eradicated enzootic bovine leukosis (EBL), which is caused by bovine leukemia virus (BLV) infection. In Serbia, efforts to eliminate EBL commenced in the late 1990s. Recognizing the disparities in test selection among laboratories and variations in quality, we evaluated the diagnostic sensitivity and specificity of commercial ELISAs using field samples in Serbia. Using 5 commercial ELISA kits, we tested 138 cattle serum samples, submitted for confirmatory testing between 2020 and 2023, along with 100 serum samples from BLV-negative herds. We found 100% agreement of the ID Screen BLV Competition (IDvet), Svanovir BLV gp51-Ab (Svanova), and INgezim BLV Compac 2.0 (Ingenasa) ELISAs. We observed 93% agreement comparing these 3 kits to the Bovine Leukemia Virus Antibody test kit (VMRD). Agreements of 92% and 88.4% were determined between Idexx and IDvet, Svanova, and Ingenasa kits, and between Idexx and VMRD kits, respectively.

查看原文本刊更多论文

检测牛白血病病毒抗体的 5 种商业 ELISA 试剂盒的性能。

为防止造成重大经济损失，一些国家已成功根除了由牛白血病病毒（BLV）感染引起的牛白血病（EBL）。在塞尔维亚，消灭 EBL 的工作始于 20 世纪 90 年代末。考虑到各实验室在检测方法选择上的差异以及检测质量的不同，我们利用塞尔维亚的现场样本对商用 ELISA 的诊断灵敏度和特异性进行了评估。我们使用 5 种商业 ELISA 试剂盒检测了在 2020 年至 2023 年期间送检的 138 份牛血清样本，以及来自 BLV 阴性牛群的 100 份血清样本。我们发现，ID Screen BLV Competition（IDvet）、Svanovir BLV gp51-Ab（Svanova）和 INgezim BLV Compac 2.0（Ingenasa）ELISAs 的一致性均为 100%。与牛白血病病毒抗体检测试剂盒（VMRD）相比，我们发现这三种试剂盒的一致性达到 93%。Idexx与IDvet、Svanova和Ingenasa试剂盒之间以及Idexx与VMRD试剂盒之间的一致性分别为92%和88.4%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Veterinary Diagnostic Investigation 农林科学-兽医学

CiteScore

3.00

自引率

6.70%

发文量

127

审稿时长

6-16 weeks

期刊介绍： The Journal of Veterinary Diagnostic Investigation (J Vet Diagn Invest) is an international peer-reviewed journal published bimonthly in English by the American Association of Veterinary Laboratory Diagnosticians (AAVLD). JVDI is devoted to all aspects of veterinary laboratory diagnostic science including the major disciplines of anatomic pathology, bacteriology/mycology, clinical pathology, epidemiology, immunology, laboratory information management, molecular biology, parasitology, public health, toxicology, and virology.