Exposure to respirable silica contributes to lower airway inflammation in asthmatic horses

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine Pub Date : 2024-10-17 DOI:10.1111/jvim.17160

Alessandra Romolo, Giulia Costa, Beatrice Sica, Giulia Memoli, Matteo Ardit, Francesco Di Benedetto, Donata Bellis, Silvana Capella, Elena Belluso, Michela Bullone

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引用次数: 0

Abstract

Background

Respirable mineral particles can induce lower airway inflammation, but the role they play in asthma of horses is unknown.

Objectives

Respirable mineral particles, particularly respirable silica, are an overlooked determinant of chronic lung inflammation (asthma) in horses.

Animals

Twenty-three horses from an equine hospital population: 11 moderately affected (MEA), 7 severely asthmatic (SEA), and 5 control horses free from respiratory clinical signs.

Methods

Prospective observational study. The quantity and quality of mineral particles found in bronchoalveolar lavage fluid (BALF) were characterized, with particular attention to silica content. Polarized light microscopy performed on cytospin slides identified intracellular birefringent particles as silica. Spectrometry-based analysis performed on whole BALF determined total mineral and silica percentage and concentration. Group-related differences in BALF mineral and silica load were investigated as well as associations with BALF cytology.

Results

Intracellular birefringent particles were increased in SEA vs MEA (median [interquartile range, IQR]), 12 [7] vs 4 [5] particles/30 high power fields [hpf], respectively; P = .01) and vs controls (4 [2] particles/30 hpf; P = .02). Total mineral concentration in BALF was similar between the groups studied, whereas silica concentration and percentage were increased in SEA vs MEA (1758 [887] particles/mL and 20 [10]% vs 867 [662] particles/mL and 8 [6]%; P = .009 and P = .001) and control group (355 [330] particles/mL and 6 [3]%; P = .0003 and P = .002). Silica load in BALF was associated with BALF neutrophilia in MEA and SEA.

Conclusions and Clinical Importance

Respirable silica is associated with neutrophilic lower airway inflammation in horses and might contribute to asthma development.

Abstract Image

查看原文本刊更多论文

接触可吸入二氧化硅会导致哮喘马的下呼吸道发炎。

背景：可吸入矿物颗粒可诱发下气道炎症，但它们在马哮喘中的作用尚不清楚：可吸入矿物颗粒，尤其是可吸入二氧化硅，是马匹慢性肺部炎症（哮喘）的一个被忽视的决定因素：动物：23 匹来自马医院的马：方法：前瞻性观察研究：方法：前瞻性观察研究。研究对支气管肺泡灌洗液（BALF）中发现的矿物质颗粒的数量和质量进行了描述，尤其关注了二氧化硅的含量。在细胞载玻片上进行的偏光显微镜检查发现细胞内的双折射颗粒为二氧化硅。对整个 BALF 进行的光谱分析确定了总矿物质和二氧化硅的百分比和浓度。研究人员还调查了各组在 BALF 矿物质和二氧化硅含量方面的差异，以及与 BALF 细胞学的关联：结果：在 SEA 与 MEA（中位数[四分位数间距，IQR]）、12 [7] 与 4 [5] 个颗粒/30 个高倍视野 [hpf]；P = .01）和对照组（4 [2] 个颗粒/30 个 hpf；P = .02）中，细胞内双折射颗粒增加。研究组之间的 BALF 总矿物质浓度相似，而 SEA 组与 MEA 组（1758 [887] 颗粒/毫升和 20 [10]% 与 867 [662] 颗粒/毫升和 8 [6]%; P = .009 和 P = .001）和对照组（355 [330] 颗粒/毫升和 6 [3]%; P = .0003 和 P = .002）相比，二氧化硅浓度和百分比均有所增加。在 MEA 和 SEA 组中，BALF 中的二氧化硅负荷与 BALF 中性粒细胞增多有关：结论和临床意义：可吸入二氧化硅与马的下气道中性粒细胞炎症有关，可能会导致哮喘的发生。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Internal Medicine 农林科学-兽医学

CiteScore

4.50

自引率

11.50%

发文量

243

审稿时长

22 weeks

期刊介绍： The mission of the Journal of Veterinary Internal Medicine is to advance veterinary medical knowledge and improve the lives of animals by publication of authoritative scientific articles of animal diseases.