Effects of morroniside isolated from Cornus officinalis fruits on functional gastrointestinal disorders and gastric ulcer in mice

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2024-10-11 DOI:10.1016/j.fitote.2024.106249

Yoshiyuki Kimura , Masahiko Taniguchi

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Abstract

The dried fruits of Cornus officinalis (Cornaceae) are used in Kampo medicine (e.g. Hachimigan and Goshajinkigan) to treat senile osteoporosis, diabetes, gastric atony, frequent urination, and diarrhea/constipation associated with aging. The present study investigated the effects of a C. officinalis fruit extract and morroniside, an iridoid compound from isolated these fruits, on the reduction in gastric emptying small intestinal motility caused by 5-hydroxytryptamine (5-HT) and 1-(3-chlorophenyl) biguanide (5-HT₃ receptor agonist), and gastric ulcers induced by 150 or 75 mM HCl/90 % EtOH (HCl-EtOH) and/or 5-HT in mice. C. officinalis extract (500 mg/kg) and morroniside (20 and 50 mg/kg) suppressed the reduction in gastric emptying induced by 5-HT- and 5-HT₃ agonist. C. officinalis extract, morroniside and 5-HT₃ receptor antagonist (ramosetron) attenuated 5-HT-induced diarrhea. Furthermore, morroniside (20 and 50 mg/kg) prevented EtOH/HCl-induced gastric ulcers and those caused by 5-HT. Morroniside (20 and 50 mg/kg) attenuated elevations in the plasma levels of corticosterone, corticotropin-releasing factor (CRF), and adrenocorticotropic hormone (ACTH) in 75 mM HCl/90 % EtOH- and 5-HT-treated mice. The results obtained herein suggest the potential of morroniside as an effective treatment for irritable bowel syndrome, such as diarrhea and functional dyspepsia (reductions in gastric emptying and small intestinal motility), caused by 5-HT. The present study suggests a role for morroniside in the regulation of elevations in CRF, ACTH, and corticosterone levels through hypothalamic-pituitary-adrenal axis activity induced by stress loading, such as a 5-HT treatment and/or HCl/EtOH stimulation.

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从山茱萸果实中分离出的山茱萸苷对小鼠功能性胃肠功能紊乱和胃溃疡的影响

山茱萸（山茱萸科）的干燥果实在堪布医药（如 Hachimigan 和 Goshajinkigan）中被用于治疗老年性骨质疏松症、糖尿病、胃失弛缓症、尿频以及与衰老有关的腹泻/便秘。本研究调查了欧当归果实提取物和morroniside（一种从这些果实中分离出来的鸢尾类化合物）对5-羟色胺（5-HT）和1-（3-氯苯基）双胍（5-HT3受体激动剂）引起的胃排空减少、150或75 mM HCl/90 % EtOH（HCl-EtOH）和/或5-HT诱发的小鼠胃溃疡的影响。欧当归提取物（500 毫克/千克）和吗罗尼苷（20 和 50 毫克/千克）可抑制 5-HT 和 5-HT3 激动剂引起的胃排空减少。欧当归提取物、吗罗尼苷和 5-HT3 受体拮抗剂（雷莫司琼）减轻了 5-HT 引起的腹泻。此外，莫罗尼甙（20 和 50 毫克/千克）还能预防乙醇/HCl 引起的胃溃疡和 5-HT 引起的胃溃疡。莫罗尼甙（20 和 50 毫克/千克）可减轻 75 mM HCl/90 % EtOH 和 5-HT 处理的小鼠血浆中皮质酮、促肾上腺皮质激素释放因子（CRF）和促肾上腺皮质激素（ACTH）水平的升高。本研究的结果表明，吗罗尼甙有可能成为治疗肠易激综合征的有效药物，如 5-HT 引起的腹泻和功能性消化不良（胃排空和小肠蠕动减少）。本研究表明，莫罗尼苷可通过应激负荷（如 5-HT 治疗和/或 HCl/EtOH 刺激）诱导的下丘脑-垂体-肾上腺轴活动，在调节 CRF、ACTH 和皮质酮水平升高方面发挥作用。

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.