Suspended Stir Bar Sorptive Extraction Based on Zr-MOF-Modified Cotton Thread for Enrichment and Detection of Three Trace Quinolones in Fish Tissue.

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

ELECTROPHORESIS Pub Date : 2024-10-14 DOI:10.1002/elps.202400096

Jiao Long, Yan Gao, Kangjia Sheng, Tao Bao, Sicen Wang

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Abstract

In this study, an efficient and practical method was established to enrich and detect three trace quinolones in fish tissue. Low-cost and environment-friendly cotton threads were coated with PCN-224 using a simple one-pot hydrothermal method. The PCN-224-modified cotton threads (PCN-224@CTs) were characterized to ensure that the crystal was successfully fabricated on the surface of cotton threads. The prepared PCN-224@CTs were wrapped around steel needles to form suspended bars. Key factors of extraction and desorption were optimized, and analytical performance was tested. Suspended bar sorptive extraction coupled with ultra-performance liquid chromatography (UPLC) with a photodiode array detector was used to analyze the quinolones in fish tissue. The detection limits of quinolones were 0.26-1.15 ng/mL, and the recoveries were achieved in the range of 81.31%-115.81%, indicating a simple method with high sensitivity and satisfactory practicability of enriching, separating, and detecting trace quinolones was successfully established.

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基于 Zr-MOF 改性棉线的悬浮搅拌棒吸附萃取法富集和检测鱼组织中的三种痕量喹诺酮类药物

本研究建立了一种高效实用的方法来富集和检测鱼组织中的三种痕量喹诺酮类药物。采用简单的一锅水热法在低成本、环保的棉线上涂覆 PCN-224。对 PCN-224 改性棉线（PCN-224@CTs）进行了表征，以确保在棉线表面成功制备晶体。制备好的 PCN-224@CTs 被缠绕在钢针上形成悬浮棒。对萃取和解吸的关键因素进行了优化，并测试了分析性能。采用悬浮条吸附萃取-超高效液相色谱（UPLC）-光电二极管阵列检测器分析鱼组织中的喹诺酮类药物。结果表明喹诺酮类药物的检出限为0.26～1.15 ng/mL，回收率为81.31%～115.81%。

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来源期刊

ELECTROPHORESIS 生物-分析化学

CiteScore

6.30

自引率

13.80%

发文量

244

审稿时长

1.9 months

期刊介绍： ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.