Downregulation of hsa_circ_0006620 inhibits the malignant progression of prostate cancer by regulation of the miR-502-3p/HK2 axis mediated by aerobic glycolysis.

IF 4.2 3区 医学 Q2 ONCOLOGY
Mingming Jin, Menghan Wang, Yulin Luo, Taile Yuan, Xue Li, Gang Huang, Qingqing Huang, Hong Sun
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Abstract

Circular RNAs (circRNA) are a class of covalently-closed, single-stranded RNAs that have been implicated in cancer progression due to their regulation of metabolism. However, the roles of circRNA in prostate cancer remain largely unknown. In this study, fluorescence in situ hybridization and RT-qPCR were used to investigate hsa_circ_0006620 expressions in both prostate cancer cells and tissues, after high-throughput sequencing. The luciferase reporter assay was used to identify hsa_circ_0006620 downstream targets. Transwell migration assays, 5-ethynyl-20-deoxyuridine assays, and Cell Counting Kit-8assays were used to investigate both proliferation and migration. In vivo tumorigenesis and metastasis assays were performed to investigate the role of hsa_circ_0006620 in prostate cancer. The results showed that hsa_circ_0006620 expression increased in prostate cancer cells and tissues. Hsa_circ_0006620 downregulation inhibited prostate cancer cell proliferation as well as in vivo and in vitro migrations. The luciferase results validated that miR-502-3p and hexokinase 2 (HK2) were hsa_circ_0006620 downstream targets. HK2 overexpression or miR-502-3p inhibition reversed prostate cancer cell migration after hsa_circ_0006620 silencing. The study also found that overexpression of HK2 or inhibition of prostate cancer reversed aerobic glycolysis after hsa_circ_0006620 silencing. In summary, the results showed thathsa_circ_0006620 downregulation inhibited prostate cancerby regulation of the miR-502-3p/HK2 axis mediated by aerobic glycolysis.

通过调节有氧糖酵解介导的 miR-502-3p/HK2 轴,下调 hsa_circ_0006620 可抑制前列腺癌的恶性进展。
环状 RNA(circRNA)是一类共价闭合的单链 RNA,由于其对新陈代谢的调控作用,已被认为与癌症进展有关。然而,circRNA 在前列腺癌中的作用在很大程度上仍然未知。本研究采用荧光原位杂交和 RT-qPCR 技术,在高通量测序后研究 hsa_circ_0006620 在前列腺癌细胞和组织中的表达。荧光素酶报告实验用于确定 hsa_circ_0006620 的下游靶点。透孔迁移测定、5-乙炔基-20-脱氧尿苷测定和细胞计数试剂盒-8测定被用来研究细胞的增殖和迁移。为了研究 hsa_circ_0006620 在前列腺癌中的作用,还进行了体内肿瘤发生和转移试验。结果表明,hsa_circ_0006620在前列腺癌细胞和组织中的表达增加。下调 Hsa_circ_0006620 可抑制前列腺癌细胞的增殖以及体内和体外迁移。荧光素酶的研究结果证实,miR-502-3p 和己糖激酶 2(HK2)是 hsa_circ_0006620 的下游靶标。过表达 HK2 或抑制 miR-502-3p 可逆转 hsa_circ_0006620 沉默后的前列腺癌细胞迁移。研究还发现,过表达 HK2 或抑制前列腺癌可逆转 hsa_circ_0006620 沉默后的有氧糖酵解。总之,研究结果表明,下调hsa_circ_0006620可通过调节有氧糖酵解介导的miR-502-3p/HK2轴抑制前列腺癌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.80
自引率
5.00%
发文量
55
审稿时长
12 months
期刊介绍: The Journal''s scope encompasses all aspects of metastasis research, whether laboratory-based, experimental or clinical and therapeutic. It covers such areas as molecular biology, pharmacology, tumor biology, and clinical cancer treatment (with all its subdivisions of surgery, chemotherapy and radio-therapy as well as pathology and epidemiology) insofar as these disciplines are concerned with the Journal''s core subject of metastasis formation, prevention and treatment.
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