A double ttrA and pduA knock-out mutant of Salmonella Typhimurium is not attenuated for mice (Mus musculus).

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Viviane Amorim Ferreira, Mauro M S Saraiva, Túlio Spina de Lima, Camila de Fátima Nascimento, Giovanna Barbosa Casagrande Paschone, André L C Rabelo, Adriana M Almeida, Oliveiro C Freitas Neto, Paul A Barrow, Angelo Berchieri Junior
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引用次数: 0

Abstract

Despite numerous studies on Salmonella enterica subsp. enterica serovar Typhimurium, the underlying mechanisms of several aspects of its virulence are still under investigation, including the role of the pdu and ttrA genes, associated with the metabolism of 1,2-propanediol using tetrathionate as an electron acceptor respectively. Our objective was to contribute to an understanding of the role of these genes inbacterial virulence for mice (Mus musculus) using an S. Typhumirum ΔttrApduA mutant. The experiment was conducted with a group infected by the S. Typhimurium mutant and a control group infected with a wild-type strain. The mutant was not attenuated compared with the parent strain. There were no differences in the bacterial numbers recovered from the mesenteric lymph nodes and Peyer's patches but at 8-day after oral infection higher numbers were recovered from the spleen, liver, and cecum. Unlike the single pduA and ttrA mutants, the double ΔttrApduA mutation did not affect invasion and survival in mice, which highlights the need for further studies to clarify the role of these important metabolism genes under reduced redox conditions linked to Salmonella virulence.

Typhimurium 沙门氏菌的双 ttrA 和 pduA 基因敲除突变体对小鼠(麝鼠)没有减毒作用。
尽管对伤寒沙门氏菌肠炎亚种进行了大量研究,但其毒力的几个方面的潜在机制仍在调查之中,包括 pdu 和 ttrA 基因的作用,这两个基因分别与使用四硫酸盐作为电子受体的 1,2 丙二醇代谢有关。我们的目的是利用酪脂菌 ΔttrApduA 突变体,帮助了解这些基因在小鼠(麝)细菌毒力中的作用。实验由感染鼠伤寒杆菌突变体的一组和感染野生型菌株的对照组进行。与亲本菌株相比,突变体没有减弱。从肠系膜淋巴结和佩耶氏斑块回收的细菌数量没有差异,但在口服感染 8 天后,从脾脏、肝脏和盲肠回收的细菌数量较高。与单 pduA 和 ttrA 突变体不同,双 ΔttrApduA 突变体不影响小鼠的侵袭和存活,这突出表明有必要进行进一步研究,以明确这些重要代谢基因在与沙门氏菌毒力相关的氧化还原条件下的作用。
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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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