Occurrence and characterization of ESKAPE organisms on the hands of veterinary students before patient contact at a veterinary academic hospital, South Africa.

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Dikeledi C Sebola, James W Oguttu, Mogaugedi N Malahlela, Marleen M Kock, Daniel N Qekwana
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引用次数: 0

Abstract

Objective: This study aimed to investigate the presence of ESKAPE organisms on the hands of students working in the intensive care unit (ICU) at a veterinary academic hospital.

Methods: A cross-sectional study was conducted among students working in an ICU at a veterinary academic hospital in South Africa. Students were sampled before the start of the ICU shift using a modified glove-juice method. Standard microbiological techniques and a series of polymerase chain reaction (PCR) assays were used to identify and characterize the bacteria. All the isolates were tested for resistance against a specific panel of antibiotics using the disk diffusion method. Proportions of bacterial species and their antimicrobial-susceptibility profiles were calculated.

Results: At screening, all the veterinary students (n = 62) carried at least one of the ESKAPE organisms on their hands. Escherichia coli was the most isolated organism (76%, 47/62), followed by P. aeruginosa (48%, 30/62), A. baumannii (47%, 29/62), E. faecium (35%, 22/62), K. pneumoniae (27%, 17/62), and S. aureus (24%, 15/62). A reduced proportion of isolates were recovered from the samples, E. coli (26%, 12/47), E. faecium (23%, 5/22), P. aeruginosa (43%, 13/30), A. baumannii (24%,7/29), K. pneumoniae (41%, 7/17), and S. aureus (20%, 3/15). Most of the organisms showed a high proportion of resistance to at least one antibiotic. Multidrug resistance was reported among just over half (56%, 5/9) of E. coli, 40% (2/5) of E. faecium, 100% (13/13) of P. aeruginosa, and 33% (1/3) of S. aureus isolates.

Conclusion: Students working in the ICU carry several organisms belonging to the ESKAPE group of organisms before contact with patients. Moreover, MDR resistance was common among this group of organisms. The findings of the present study underscore the importance of infection prevention and control (IPC) strategies to help reduce the likelihood of the spread of these organisms to personnel, owners, family members, and patients.

南非一家兽医学术医院的兽医专业学生在接触病人之前手部出现 ESKAPE 微生物的情况及特征。
研究目的本研究旨在调查在一家兽医学术医院重症监护室(ICU)工作的学生手上是否存在ESKAPE微生物:对在南非一家兽医学术医院重症监护室工作的学生进行了一项横断面研究。在重症监护室轮班开始前,使用改良的手套汁液法对学生进行采样。采用标准微生物学技术和一系列聚合酶链反应 (PCR) 检测方法对细菌进行鉴定和定性。使用磁盘扩散法检测了所有分离菌对特定抗生素的耐药性。计算细菌种类比例及其抗菌药敏感性:在筛查时,所有兽医专业学生(n = 62)的手上至少携带一种 ESKAPE 细菌。大肠埃希菌是分离最多的微生物(76%,47/62),其次是铜绿假单胞菌(48%,30/62)、鲍曼不动杆菌(47%,29/62)、粪大肠杆菌(35%,22/62)、肺炎双球菌(27%,17/62)和金黄色葡萄球菌(24%,15/62)。从样本中回收的分离菌比例较低,分别为大肠杆菌(26%,12/47)、粪大肠杆菌(23%,5/22)、铜绿假单胞菌(43%,13/30)、鲍曼不动杆菌(24%,7/29)、肺炎双球菌(41%,7/17)和金黄色葡萄球菌(20%,3/15)。大多数微生物对至少一种抗生素的耐药性比例较高。超过一半(56%,5/9)的大肠杆菌、40%(2/5)的粪大肠杆菌、100%(13/13)的铜绿假单胞菌和 33%(1/3)的金黄色葡萄球菌分离物对多种药物产生了耐药性:结论:在重症监护室工作的学生在与病人接触前会携带几种属于 ESKAPE 菌群的微生物。结论:在重症监护室工作的学生在接触病人之前会携带几种属于 ESKAPE 菌群的微生物,而且这类微生物普遍存在 MDR 耐药性。本研究的结果凸显了感染预防和控制(IPC)策略的重要性,有助于降低这些微生物向工作人员、业主、家庭成员和患者传播的可能性。
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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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