Improved catalytic stability of immobilized Candida antarctica lipase B on macroporous resin with organic polymer coating for biodiesel production.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioprocess and Biosystems Engineering Pub Date : 2024-10-22 DOI:10.1007/s00449-024-03099-w

Jiale Liu, Shufan Zhao, Wan Wei, Shupeng Yu, Zhao Wang, Jianyong Zheng

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引用次数: 0

Abstract

Lipase is one of the most widely studied and applied biocatalysts. Due to the high enzyme leakage rate of the immobilization method of physical adsorption, we propose a new lipase immobilization method, based on the combination of macroporous resin adsorption and organic polymer coating. The immobilized Candida antarctica lipase B (CALB@resin-CAB) was prepared by combining the macroporous resin adsorption with cellulose acetate butyrate coating, and its structure was characterized by various analytic methods. Immobilized lipase was applied for biodiesel production using acidified palm oil as the starting material, the conversion rate achieved as high as 98.5% in two steps. Furthermore, the immobilized lipase displayed satisfactory stability and reusability in biodiesel production. When the aforementioned reaction was carried out in a continuous flow packed bed system, the yield of biodiesel was 94.8% and space-time yield was 2.88 g/(mL∙h). The immobilized lipase CALB@resin-CAB showed high catalytic activity and stability, which has good potential for industrial application in the field of oil processing.

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提高大孔树脂上固定化白色念珠菌脂肪酶 B 的催化稳定性，使其具有有机聚合物涂层，用于生物柴油生产。

脂肪酶是研究和应用最广泛的生物催化剂之一。由于物理吸附固定化方法的酶泄漏率较高，我们提出了一种基于大孔树脂吸附和有机聚合物包覆相结合的新型脂肪酶固定化方法。通过大孔树脂吸附与醋酸纤维素丁酸酯包覆相结合的方法制备了固定化白色念珠菌脂肪酶 B（CALB@resin-CAB），并通过多种分析方法对其结构进行了表征。固定化脂肪酶被用于以酸化棕榈油为起始原料生产生物柴油，两步转化率高达 98.5%。此外，固定化脂肪酶在生物柴油生产中表现出令人满意的稳定性和可重复使用性。在连续流填料床系统中进行上述反应时，生物柴油的产量为 94.8%，时空产量为 2.88 g/（mL∙h）。固定化脂肪酶 CALB@resin-CAB 具有较高的催化活性和稳定性，在油脂加工领域具有良好的工业应用前景。

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.