Stimulated Brillouin scattering flow cytometry.

IF 2.9 2区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Biomedical optics express Pub Date : 2024-09-25 eCollection Date: 2024-10-01 DOI:10.1364/BOE.537602

Jake R Rosvold, Giulia Zanini, Chenchen Handler, Eric Frank, Jiarui Li, Michele I Vitolo, Stuart S Martin, Giuliano Scarcelli

引用次数: 0

Abstract

We present the use of stimulated Brillouin scattering spectroscopy to achieve rapid measurements of cell biomechanics in a flow cytometer setup. Specifically, our stimulated Brillouin scattering flow cytometry can acquire at a rate of 200 Hz, with a spectral acquisition time of 5 ms, which marks a 10x improvement compared to previous demonstrations of spontaneous Brillouin scattering flow cytometry. We experimentally validate our stimulated Brillouin scattering flow cytometer by measuring cell populations of normal breast epithelial cells and metastatic breast epithelial cancer cells.

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受激布里渊散射流式细胞仪。

我们介绍了在流式细胞仪设置中使用受激布里渊散射光谱快速测量细胞生物力学的方法。具体来说，我们的受激布里渊散射流式细胞仪能以 200 Hz 的速率采集，光谱采集时间为 5 毫秒，与之前展示的自发布里渊散射流式细胞仪相比，提高了 10 倍。我们通过测量正常乳腺上皮细胞和转移性乳腺上皮癌细胞的细胞群，对刺激布里渊散射流式细胞仪进行了实验验证。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical optics express BIOCHEMICAL RESEARCH METHODS-OPTICS

CiteScore

6.80

自引率

11.80%

发文量

633

审稿时长

1 months

期刊介绍： The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including: Tissue optics and spectroscopy Novel microscopies Optical coherence tomography Diffuse and fluorescence tomography Photoacoustic and multimodal imaging Molecular imaging and therapies Nanophotonic biosensing Optical biophysics/photobiology Microfluidic optical devices Vision research.