Geranylgeranylated-chlorophyll-protein complexes in lhl3 mutant of the green alga Chlamydomonas reinhardtii

IF 6.2 1区 生物学 Q1 PLANT SCIENCES
Sireesha Kodru, Sreedhar Nellaepalli, Shin-Ichiro Ozawa, Chihiro Satoh, Hiroshi Kuroda, Ryouichi Tanaka, Katharine Guan, Marilyn Kobayashi, Phoi Tran, Sarah McCarthy, Setsuko Wakao, Krishna K. Niyogi, Yuichiro Takahashi
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引用次数: 0

Abstract

Chlorophylls a and b (Chl a and b) are involved in light harvesting, photochemical reactions, and electron transfer reactions in plants and green algae. The core complexes of the photosystems (PSI and PSII) associate with Chl a, while the peripheral antenna complexes (LHCI and LHCII) bind Chls a and b. One of the final steps of Chl biosynthesis is the conversion of geranylgeranylated Chls (ChlsGG) to phytylated Chls by geranylgeranyl reductase (GGR). Here, we isolated and characterized a pale green mutant of the green alga Chlamydomonas reinhardtii that was very photosensitive and was unable to grow photoautotrophically. This mutant has a 16-bp deletion in the LHL3 gene, which resulted in the loss of LHL3 and GGR and accumulated only ChlsGG. The lhl3 mutant cells grown in the dark accumulated PSII and PSI proteins at 25–50% of WT levels, lacked PSII activity, and retained a decreased PSI activity. The PSII and PSI proteins were depleted to trace amounts in the mutant cells grown in light. In contrast, the accumulation of LHCI and LHCII was unaffected except for LHCA3. Our results suggest that the replacement of Chls with ChlsGG strongly affects the structural and functional integrity of PSII and PSI complexes but their associating LHC complexes to a lesser extent. Affinity purification of HA-tagged LHL3 confirmed the formation of a stable LHL3-GGR complex, which is vital for GGR stability. The LHL3-GGR complex contained a small amount of PSI complex assembly factors, suggesting a putative coupling between Chl synthesis and PSI complex assembly.

Abstract Image

绿色藻类莱茵衣藻 lhl3 突变体中的叶绿素-蛋白质复合物。
叶绿素 a 和 b(Chl a 和 b)参与植物和绿藻的光收集、光化学反应和电子传递反应。叶绿素生物合成的最后步骤之一是通过精氨酰精氨酰还原酶(GGR)将精氨酰精氨酰化叶绿素(ChlsGG)转化为植物tyl化叶绿素。在这里,我们分离并鉴定了一种淡绿色突变体,这种突变体对光非常敏感,无法进行光能自养生长。该突变体的 LHL3 基因缺失了 16 个 bp,导致 LHL3 和 GGR 的缺失,只积累了 ChlsGG。在黑暗中生长的 lhl3 突变体细胞积累的 PSII 和 PSI 蛋白只有 WT 水平的 25-50%,缺乏 PSII 活性,PSI 活性降低。在光下生长的突变体细胞中,PSII 和 PSI 蛋白被消耗到微量。相反,除了 LHCA3 外,LHCI 和 LHCII 的积累未受影响。我们的结果表明,用 ChlsGG 替代 Chls 会强烈影响 PSII 和 PSI 复合物的结构和功能完整性,但其关联的 LHC 复合物受到的影响较小。HA标记的LHL3的亲和纯化证实了稳定的LHL3-GGR复合物的形成,这对GGR的稳定性至关重要。LHL3-GGR 复合物含有少量 PSI 复合物组装因子,这表明 Chl 合成与 PSI 复合物组装之间可能存在耦合。
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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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