The receptor-like cytoplasmic kinase OsBSK1-2 regulates immunity via an HLH/bHLH complex

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Xun Wang, Zhijuan Diao, Chang Cao, Yan Liu, Na Xia, Youlian Zhang, Ling Lu, Fanyu Kong, Houli Zhou, Lizhe Chen, Jing Zhang, Bangsheng Wang, Ronghua Huang, Dingzhong Tang, Shengping Li
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引用次数: 0

Abstract

Plants need to fine-tune defense responses to maintain a robust but flexible host barrier to various pathogens. Helix-loop-helix/basic helix-loop-helix (HLH/bHLH) complexes play important roles in fine-tuning plant development. However, the function of these genes in plant immunity and how they are regulated remain obscure. Here, we identified an atypical bHLH transcription factor, Oryza sativa (Os)HLH46, that interacts with rice receptor-like cytoplasmic kinase (RLCK) Os BRASSINOSTEROID-SIGNALING KINASE1-2 (OsBSK1-2), which plays a key role in rice blast resistance. OsBSK1-2 stabilized OsHLH46 both in vivo and in vitro. In addition, OsHLH46 positively regulates rice blast resistance, which depends on OsBSK1-2. OsHLH46 has no transcriptional activation activity and interacts with a typical bHLH protein, OsbHLH6, which negatively regulates rice blast resistance. OsbHLH6 binds to the promoter of OsWRKY45 and inhibits its expression, while OsHLH46 suppresses the function of OsbHLH6 by blocking its DNA binding and transcriptional inhibition of OsWRKY45. Consistent with these findings, OsWRKY45 was up-regulated in OsHLH46-overexpressing plants. In addition, the oshlh46 mutant overexpressing OsbHLH6 is more susceptible to Magnaporthe oryzae than is the wild type, suggesting that OsHLH46 suppresses OsbHLH6-mediated rice blast resistance. Our results not only demonstrated that OsBSK1-2 regulates rice blast resistance via the OsHLH46/OsbHLH6 complex, but also uncovered a new mechanism for plants to fine-tune plant immunity by regulating the HLH/bHLH complex via RLCKs.

Abstract Image

受体样细胞质激酶 OsBSK1-2 通过 HLH/bHLH 复合物调节免疫。
植物需要对防御反应进行微调,以保持强大而灵活的宿主屏障,抵御各种病原体的侵袭。螺旋-环-螺旋/基本螺旋-环-螺旋(HLH/bHLH)复合体在微调植物发育过程中发挥着重要作用。然而,这些基因在植物免疫中的功能及其调控方式仍不清楚。在这里,我们发现了一种非典型 bHLH 转录因子 Oryza sativa (Os)HLH46 与水稻受体样细胞质激酶(RLCK)Os BRASSINOSTEROID-SIGNALING KINASE1-2 (OsBSK1-2)相互作用,后者在水稻抗稻瘟病中起着关键作用。OsBSK1-2 在体内和体外都能稳定 OsHLH46。此外,OsHLH46对稻瘟病抗性有正向调节作用,而稻瘟病抗性的产生有赖于OsBSK1-2。OsHLH46 没有转录激活活性,它与典型的 bHLH 蛋白 OsbHLH6 相互作用,后者对稻瘟病抗性有负向调节作用。OsbHLH6 与 OsWRKY45 的启动子结合并抑制其表达,而 OsHLH46 则通过阻断其 DNA 结合和对 OsWRKY45 的转录抑制作用来抑制 OsbHLH6 的功能。与这些发现一致的是,OsHLH46过表达植株中OsWRKY45上调。此外,过表达 OsbHLH6 的 oshlh46 突变体比野生型更易感染 Magnaporthe oryzae,这表明 OsHLH46 抑制了 OsbHLH6 介导的稻瘟病抗性。我们的研究结果不仅证明了OsBSK1-2通过OsHLH46/OsbHLH6复合物调控稻瘟病抗性,而且揭示了植物通过RLCKs调控HLH/bHLH复合物微调植物免疫的新机制。
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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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