Developing polycistronic expression tool in Yarrowia lipolytica

IF 4.4 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Synthetic and Systems Biotechnology Pub Date : 2024-09-28 DOI:10.1016/j.synbio.2024.09.010

Donghan Li , Jianhui Liu , Lingxuan Sun , Jin Zhang , Jin Hou

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引用次数: 0

Abstract

Unconventional oleaginous yeast Yarrowia lipolytica has gained widespread applications as a microbial cell factory for synthesizing various chemicals and natural products. The construction of efficient cell factories requires intricate metabolic engineering. However, multi-gene expression in Y. lipolytica is labor-intensive. To facilitate multi-gene expression, we developed the polycistronic expression tool using 2A peptides. We first compared different 2A peptides in Y. lipolytica and identified two 2A peptides with high cleavage efficiency: P2A and ERBV-1. The effect of 2A peptides on the expression level of upstream and downstream genes was then determined. Ultimately, we applied the identified 2A peptides to express four genes in canthaxanthin biosynthetic pathway within one expression cassette for canthaxanthin production. This study enriches the multi-gene expression tools of Y. lipolytica, which will facilitate the cell factory construction of Y. lipolytica.

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在脂溶性亚罗酵母中开发多聚组蛋白表达工具

非常规油脂酵母亚罗酵母（Yarrowia lipolytica）作为合成各种化学品和天然产品的微生物细胞工厂，已获得广泛应用。构建高效细胞工厂需要复杂的代谢工程。然而，在脂溶性酵母中进行多基因表达是一项劳动密集型工作。为了促进多基因表达，我们利用 2A 肽开发了多聚组表达工具。我们首先比较了脂溶性酵母中不同的 2A 肽，确定了两种裂解效率较高的 2A 肽：P2A和ERBV-1。然后确定了 2A 肽对上游和下游基因表达水平的影响。最终，我们利用所鉴定的 2A 肽在一个表达盒中表达了角黄素生物合成途径中的四个基因，以生产角黄素。这项研究丰富了溶脂酵母的多基因表达工具，有助于溶脂酵母细胞工厂的构建。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Synthetic and Systems Biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-

CiteScore

6.90

自引率

12.50%

发文量

审稿时长

67 days

期刊介绍： Synthetic and Systems Biotechnology aims to promote the communication of original research in synthetic and systems biology, with strong emphasis on applications towards biotechnology. This journal is a quarterly peer-reviewed journal led by Editor-in-Chief Lixin Zhang. The journal publishes high-quality research; focusing on integrative approaches to enable the understanding and design of biological systems, and research to develop the application of systems and synthetic biology to natural systems. This journal will publish Articles, Short notes, Methods, Mini Reviews, Commentary and Conference reviews.