Investigating the effects of COVID-19 on sperm in male smokers: A prospective integrated proteomic and metabolomic study

IF 3.3 4区医学 Q2 REPRODUCTIVE BIOLOGY

Reproductive toxicology Pub Date : 2024-10-13 DOI:10.1016/j.reprotox.2024.108734

ChengLu Wang , JiaCheng Zhang , Fang Gao , Min Zheng , XiaoHua Fu , KeBing Yang

{"title":"Investigating the effects of COVID-19 on sperm in male smokers: A prospective integrated proteomic and metabolomic study","authors":"ChengLu Wang , JiaCheng Zhang , Fang Gao , Min Zheng , XiaoHua Fu , KeBing Yang","doi":"10.1016/j.reprotox.2024.108734","DOIUrl":null,"url":null,"abstract":"<div><div>Notable variations in semen parameters among non-smoking males have been documented post-COVID-19 pandemic. The role of smoking as a significant contributing factor to male infertility has been substantiated. Does the combined effect of smoking and SARS-CoV-2 infection impact male reproductive function? A prospective descriptive cohort study was performed using data from 90 smoking and 90 non-smoking males before and after coronavirus infection in a single center over a period of 3 months. Semen samples were collected before and within 15 days after COVID-19 infection, ensuring no more than three months elapsed between the two collections. The semen parameters evaluated included volume, concentration, progressive motility, normal morphology, and DNA fragmentation rate. Proteomic and metabolomic studies were further used to explore the differences between groups. Both non-smokers and smokers exhibited a marked reduction in sperm concentration, progressive motility, and normal morphology rate. Additionally, an increase in sperm DNA fragmentation index was noted for non-smokers and smokers. In the non-smoking group, dysregulation proteins including SEMG1, SEMG2 and DNAH5, and metabolites including L-glutamine, cis-9-Palmitoleic acid and Linoleamide were observed. In smokers, dysregulation proteins including SMCP, ROPN1B and IZUMO4, alongside metabolites including carnitine, gamma-Glutamylglutamic acid, and hypoxanthine were found. Comparative analysis between smoking and non-smoking patients post-COVID-19 also revealed significant differences in semen concentration, morphology and sperm DNA fragmentation rate. Dysregulated proteins including HSPA5, HSPA2 and PGK2, and metabolites such as acetylcarnitine, oxaloacetate and nicotinate were associated with impaired sperm function. Our study demonstrates that the virus also significantly compromises sperm quality in smoking males, who experience more pronounced declines post-infection compared to their non-smoking counterparts. This research underscores the necessity for comprehensive fertility assessments for smoking males after recovering from COVID-19.</div></div>","PeriodicalId":21137,"journal":{"name":"Reproductive toxicology","volume":"130 ","pages":"Article 108734"},"PeriodicalIF":3.3000,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reproductive toxicology","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0890623824002016","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"REPRODUCTIVE BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Notable variations in semen parameters among non-smoking males have been documented post-COVID-19 pandemic. The role of smoking as a significant contributing factor to male infertility has been substantiated. Does the combined effect of smoking and SARS-CoV-2 infection impact male reproductive function? A prospective descriptive cohort study was performed using data from 90 smoking and 90 non-smoking males before and after coronavirus infection in a single center over a period of 3 months. Semen samples were collected before and within 15 days after COVID-19 infection, ensuring no more than three months elapsed between the two collections. The semen parameters evaluated included volume, concentration, progressive motility, normal morphology, and DNA fragmentation rate. Proteomic and metabolomic studies were further used to explore the differences between groups. Both non-smokers and smokers exhibited a marked reduction in sperm concentration, progressive motility, and normal morphology rate. Additionally, an increase in sperm DNA fragmentation index was noted for non-smokers and smokers. In the non-smoking group, dysregulation proteins including SEMG1, SEMG2 and DNAH5, and metabolites including L-glutamine, cis-9-Palmitoleic acid and Linoleamide were observed. In smokers, dysregulation proteins including SMCP, ROPN1B and IZUMO4, alongside metabolites including carnitine, gamma-Glutamylglutamic acid, and hypoxanthine were found. Comparative analysis between smoking and non-smoking patients post-COVID-19 also revealed significant differences in semen concentration, morphology and sperm DNA fragmentation rate. Dysregulated proteins including HSPA5, HSPA2 and PGK2, and metabolites such as acetylcarnitine, oxaloacetate and nicotinate were associated with impaired sperm function. Our study demonstrates that the virus also significantly compromises sperm quality in smoking males, who experience more pronounced declines post-infection compared to their non-smoking counterparts. This research underscores the necessity for comprehensive fertility assessments for smoking males after recovering from COVID-19.

查看原文本刊更多论文

调查 COVID-19 对男性吸烟者精子的影响：前瞻性蛋白质组和代谢组综合研究

据记录，COVID-19 大流行后，不吸烟男性的精液参数出现了明显的变化。吸烟是导致男性不育的一个重要因素已得到证实。吸烟和 SARS-CoV-2 感染共同作用是否会影响男性生殖功能？一项前瞻性描述性队列研究使用了一个中心的 90 名吸烟男性和 90 名非吸烟男性在冠状病毒感染前后 3 个月内的数据。在感染 COVID-19 之前和之后的 15 天内采集精液样本，确保两次采集间隔不超过三个月。评估的精液参数包括精液量、精液浓度、精子活力、正常形态和DNA碎片率。蛋白质组学和代谢组学研究进一步用于探究组间差异。非吸烟者和吸烟者的精子浓度、活动力和正常形态率都明显下降。此外，非吸烟者和吸烟者的精子 DNA 碎片指数都有所增加。在非吸烟组中，观察到包括 SEMG1、SEMG2 和 DNAH5 在内的蛋白质以及包括 L-谷氨酰胺、顺式-9-棕榈油酸和亚油酰胺在内的代谢物失调。在吸烟者中，发现了包括 SMCP、ROPN1B 和 IZUMO4 在内的失调蛋白，以及包括肉碱、γ-谷氨酰谷氨酸和次黄嘌呤在内的代谢物。对COVID-19后吸烟和不吸烟患者的比较分析还显示，精液浓度、形态和精子DNA碎片率存在显著差异。HSPA5、HSPA2 和 PGK2 等失调蛋白以及乙酰肉碱、草酰乙酸和烟酸等代谢物与精子功能受损有关。我们的研究表明，病毒也会严重损害吸烟男性的精子质量，与不吸烟的男性相比，吸烟男性的精子质量在感染病毒后会出现更明显的下降。这项研究强调了对 COVID-19 病毒康复后的吸烟男性进行全面生育能力评估的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Reproductive toxicology 生物-毒理学

CiteScore

6.50

自引率

3.00%

发文量

131

审稿时长

45 days

期刊介绍： Drawing from a large number of disciplines, Reproductive Toxicology publishes timely, original research on the influence of chemical and physical agents on reproduction. Written by and for obstetricians, pediatricians, embryologists, teratologists, geneticists, toxicologists, andrologists, and others interested in detecting potential reproductive hazards, the journal is a forum for communication among researchers and practitioners. Articles focus on the application of in vitro, animal and clinical research to the practice of clinical medicine. All aspects of reproduction are within the scope of Reproductive Toxicology, including the formation and maturation of male and female gametes, sexual function, the events surrounding the fusion of gametes and the development of the fertilized ovum, nourishment and transport of the conceptus within the genital tract, implantation, embryogenesis, intrauterine growth, placentation and placental function, parturition, lactation and neonatal survival. Adverse reproductive effects in males will be considered as significant as adverse effects occurring in females. To provide a balanced presentation of approaches, equal emphasis will be given to clinical and animal or in vitro work. Typical end points that will be studied by contributors include infertility, sexual dysfunction, spontaneous abortion, malformations, abnormal histogenesis, stillbirth, intrauterine growth retardation, prematurity, behavioral abnormalities, and perinatal mortality.