JAK/STAT signaling pathway is involved in antibacterial immunity in the green peach aphid Myzus persicae (Sulzer)

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology Pub Date : 2024-10-09 DOI:10.1016/j.pestbp.2024.106168

Bo Ding , Chao Zhang , Li He , Qinghui Zeng , Senwen Zhang , Hanzhi Yang , Hong Yang

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Abstract

The Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway within the innate immune system plays a crucial role in defending insects against bacterial, fungal, and viral pathogens. In this study, we identified and cloned five key genes of this signaling pathway in Myzus persicae: MpDome-1, MpDome-2, MpJak, MpStat92E-1, and MpStat92E-2. Our results illustrated that these genes were highly expressed in first, second and third-instar nymphs. Tissue-specific expression analysis revealed that the five genes were predominantly expressed in the gut. Upon bacterial challenge, particularly with Staphylococcus aureus, the expression levels of all five genes were significantly upregulated. Additionally, Escherichia coli infection significantly upregulated the expression levels of MpDome-1 and MpDome-2, while MpJak, MpStat92E-1 and MpStat92E-2 were weakly upregulated. Functional analysis through RNA interference-mediated knockdown of these target genes revealed a significant increase in mortality following infection with E. coli and S. aureus compared with the control group. These findings suggest that the JAK/STAT signaling pathway is crucial for immune defense against bacterial infections in M. persicae.

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JAK/STAT 信号通路参与了桃蚜 Myzus persicae (Sulzer) 的抗菌免疫过程

先天性免疫系统中的 Janus 激酶/信号转导和转录激活因子（JAK/STAT）信号通路在昆虫抵御细菌、真菌和病毒病原体的过程中发挥着至关重要的作用。在这项研究中，我们发现并克隆了柿小蜂（Myzus persicae）中该信号通路的五个关键基因：MpDome-1、MpDome-2、MpJak、MpStat92E-1 和 MpStat92E-2。我们的研究结果表明，这些基因在一龄、二龄和三龄若虫中的表达量很高。组织特异性表达分析表明，这五个基因主要在肠道中表达。在细菌挑战下，特别是在金黄色葡萄球菌的挑战下，所有五个基因的表达水平都显著上调。此外，大肠杆菌感染会显著上调MpDome-1和MpDome-2的表达水平，而MpJak、MpStat92E-1和MpStat92E-2的上调较弱。通过 RNA 干扰介导的这些靶基因敲除功能分析发现，与对照组相比，感染大肠杆菌和金黄色葡萄球菌后死亡率显著增加。这些研究结果表明，JAK/STAT 信号通路对宿主对细菌感染的免疫防御至关重要。

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来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.