External nutrients fattening promotes hepatopancreases development, metabolism, nutrient deposition and flavor yield during the maturation stage in female Chinese mitten crab (Eriocheir sinensis)

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2024-10-15 DOI:10.1016/j.aqrep.2024.102428

Changyou Song , Jianxiang Chen , Jiyan He , Miaomiao Xue , Pao Xu , Huiming Ding , Ningyuan Wu , Ruicheng Zhao , Gangchun Xu , Hongxia Li

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引用次数: 0

Abstract

The nutritive value and maturity status of hepatopancreases dominates the quality of Chinese mitten crab, Eriocheir sinensis. However, the underlying function and regulatory mechanism, as well as the external nutrient factors on the development of hepatopancreases in different growth stages of E. sinensis remains unclear. In this study, we first revealed the function of hepatopancreases feeding with iced trash fish (ITF) during the whole culture period; and further investigated the underlying mechanism on nutrient deposition and flavor yield feeding with formulated feed (FEED) in replacement of ITF in female E. sinensis. From the results, growth performance and metabolism indicate E. sinensis was robustly developed, and ITF promotes growth and hepatopancreases development during the maturation period. Meanwhile, activation of fatty acid and amino acid metabolism in hepatopancreases resulted in depletion of monounsaturated fatty acids (MUFAs) but promoted protein deposition during the maturation stage. Integrated analysis of transcriptome and metabolome reveal metabolism, biosynthesis, and flavor presentation were intensively active during maturation, evidenced by the expression of key genes of ALDH, TBXAs, HADHA, UPB1, CYP2, GST, and γ-GT-X1; as well as the key metabolites of GMP and IMP. Additionally, substitution of ITF with FEED further confirm full price artificial feed could meet the nutrient requirements during the maturation period, evidenced by the growth performance, fatty acid and amino acid derived nutrient and flavor yield in hepatopancreases. In conclusion, nutrient and flavor deposition was associated with the fast development of hepatopancreases, and fatten with full price compound feed contributes to the nutrient requirement and flavor yield during the maturation stage.

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外源营养肥育促进中华绒螯蟹雌蟹成熟期肝胰腺发育、代谢、营养沉积和产卵率

肝胰腺的营养价值和成熟度对中华绒螯蟹的品质起着决定性作用。然而，中华绒螯蟹肝胰腺在不同生长阶段的基本功能、调控机制以及外部营养因素对其发育的影响仍不清楚。在本研究中，我们首先揭示了在整个培养期间投喂冰杂鱼（ITF）的肝胰腺的功能，并进一步研究了用配方饲料（FEED）替代 ITF 投喂对雌性中华绒螯虾营养沉积和风味产量的内在机制。结果表明，中华绒螯虾的生长性能和新陈代谢旺盛，ITF能促进成熟期中华绒螯虾的生长和肝胰腺的发育。同时，肝胰腺中脂肪酸和氨基酸代谢的激活导致了单不饱和脂肪酸（MUFAs）的消耗，但促进了成熟期蛋白质的沉积。对转录组和代谢组的综合分析表明，成熟期的代谢、生物合成和风味呈现非常活跃，ALDH、TBXAs、HADHA、UPB1、CYP2、GST 和 γ-GT-X1 等关键基因以及 GMP 和 IMP 等关键代谢物的表达就是证明。此外，用 FEED 替代 ITF 进一步证实了全价人工饲料可以满足成熟期的营养需求，这一点可以从生长性能、肝胰脏中脂肪酸和氨基酸衍生营养和风味的产量得到证明。总之，营养物质和风味物质的沉积与肝胰腺的快速发育有关，使用全价配合饲料育肥有助于满足肝胰腺成熟期的营养需求和风味产量。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.