A Novel Fluorescent/Colorimetric Dual-Signal Intelligent Detecting Platform (F/C-BMIPs) Based on “Borate-Molecular Imprinting” Collaborative Recognition for Visual Quantitative Detection of Rutin in Food Samples

IF 3.2 4区 化学 Q2 CHEMISTRY, ANALYTICAL
Luminescence Pub Date : 2024-10-14 DOI:10.1002/bio.4926
Lei Zhang, Xueying Niu, Zhenmin Yan, Chang Liu, Yu Zhang, Peiting Wang, Milan Wang
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引用次数: 0

Abstract

Herein, a novel fluorescent/colorimetric dual-signal intelligent detecting platform (F/C-BMIPs) based on ratiometric fluorescence (BA-N-GQDs/rhodamine 6G) and collaborative recognition (borate-MIPs) was developed for on-site visual quantitative detection of rutin (RT) in food samples. Furthermore, the above detection system is transferred to the test strips, and combined with the color recognition ability of smartphones, the portable and user-friendly visualization and quantitative detection of RT (F/C-BMIPs method) is realized. Under optimal conditions, the assay system has a wide linear range of 0.2–10 μM (F-BMIPs)/0.45–10 μM (F/C-BMIPs), and 10–100 μM (F-BMIPs and F/C-BMIPs), a detection limit as low as 0.02 μM (F-BMIPs)/0.056 μM (F/C-BMIPs) (S/N = 3), highly imprinted factor (IF = 5.04), and fast fluorescence response (90 s). In addition, this method was successfully applied to the detection of RT in two real samples (raw buckwheat and Sophora japonica), and the recovery rate was 95.6%–103.5%. Therefore, this study provides a promising strategy for the on-site rapid detection of cis-diol-containing flavonoids in food samples.

基于 "硼酸盐-分子印迹 "协同识别的新型荧光/比色双信号智能检测平台 (F/C-BMIPs),用于食品样品中芦丁的目视定量检测
本文开发了一种基于比率荧光(BA-N-GQDs/罗丹明6G)和协同识别(硼酸盐-MIPs)的新型荧光/比色双信号智能检测平台(F/C-BMIPs),用于现场可视化定量检测食品样品中的芦丁(RT)。此外,将上述检测系统移植到试纸上,结合智能手机的颜色识别能力,实现了便携式、人性化的可视化定量检测芦丁(F/C-BMIPs 法)。在最佳条件下,该检测系统具有 0.2-10 μM(F-BMIPs)/0.45-10 μM(F/C-BMIPs)和 10-100 μM(F-BMIPs 和 F/C-BMIPs)的宽线性范围,检测限低至 0.02 μM(F-BMIPs)/0.056 μM(F/C-BMIPs)(S/N = 3)、高印记因子(IF = 5.04)和快速荧光响应(90 秒)。此外,该方法还成功应用于两种实际样品(生荞麦和槐花)中 RT 的检测,回收率为 95.6%-103.5%。因此,该研究为现场快速检测食品样品中含有的顺式二醇类黄酮提供了一种可行的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Luminescence
Luminescence 生物-生化与分子生物学
CiteScore
5.10
自引率
13.80%
发文量
248
审稿时长
3.5 months
期刊介绍: Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry. Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.
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