High soybean dietary supplementation with quercetin improves antioxidant capacity of spotted sea bass Lateolabrax maculatus

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2024-10-14 DOI:10.1016/j.aqrep.2024.102429

Longhui Liu , Yanbo Zhao , Zhangfan Huang , Zhongying Long , Huihui Qin , Hao Lin , Sishun Zhou , Lumin Kong , Jianrong Ma , Yi Lin , Zhongbao Li

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Abstract

This study aimed to examine the effect of quercetin (QUE) on the antioxidant capacity of spotted sea bass that were fed a high soybean meal diet. 44 % fish meal was used as a healthy control, and soybean meal instead of 50 % fish meal induced a negative reaction. On this basis, 0.25, 0.50, 0.75, and 1.00 g/kg QUE were added, respectively. A total of 540 spotted sea bass were randomly allocated into 6 groups and provided with diets that corresponded to their respective groups for a duration of 56 days. The results showed a significant decrease in the antioxidant capacity of spotted sea bass fed a high soybean meal diet. The inclusion of QUE considerably improved the antioxidant capacity of spotted sea bass. This was evidenced by the notable rise in glutathione (GSH) levels in the intestine and liver, as well as the elevated levels of catalase (CAT) and total antioxidant capacity (T-AOC) in the liver. Furthermore, transcriptome results showed that the S-transferase (GST) gene in the glutathione metabolism pathway was significantly down-regulated by high soybean meal, while with the addition of QUE, noteworthy alterations in genes associated with the PI3K-Akt signaling pathway, Foxo signaling pathway, and AMPK signaling pathway. These signaling pathways are involved in signal transduction, apoptosis, and oxidative stress. On the other hand, genes related to energy metabolism, such as phosphoenolpyruvate carboxykinase (PEPCK), were observed to be upregulated. These pathways could potentially contribute to the antioxidant properties of QUE.

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大豆膳食补充槲皮素可提高斑点叉尾鲈的抗氧化能力

本研究旨在考察槲皮素（QUE）对以高豆粕为食的斑点海鲈抗氧化能力的影响。44 % 的鱼粉被用作健康对照，而豆粕代替 50 % 的鱼粉会引起不良反应。在此基础上，分别添加 0.25、0.50、0.75 和 1.00 g/kg QUE。将 540 尾斑点叉尾鲈随机分为 6 组，并提供与各组相应的日粮，持续 56 天。结果显示，饲喂高豆粕日粮的斑海鲈抗氧化能力明显下降。加入 QUE 后，斑海鲈的抗氧化能力大大提高。肠道和肝脏中谷胱甘肽（GSH）水平的显著上升，以及肝脏中过氧化氢酶（CAT）和总抗氧化能力（T-AOC）水平的提高都证明了这一点。此外，转录组结果显示，谷胱甘肽代谢途径中的S-转移酶（GST）基因在高豆粕的作用下显著下调，而添加QUE后，与PI3K-Akt信号通路、Foxo信号通路和AMPK信号通路相关的基因发生了显著变化。这些信号通路涉及信号转导、细胞凋亡和氧化应激。另一方面，与能量代谢有关的基因，如磷酸烯醇丙酮酸羧激酶（PEPCK），被观察到上调。这些途径可能有助于提高 QUE 的抗氧化特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.